Polybasic Patches in Both C2 Domains of Synaptotagmin-1 Are Required for Evoked Neurotransmitter Release
Wu Z, Ma L, Courtney NA, Zhu J, Landajuela A, Zhang Y, Chapman ER, Karatekin E. Polybasic Patches in Both C2 Domains of Synaptotagmin-1 Are Required for Evoked Neurotransmitter Release. Journal Of Neuroscience 2022, 42: 5816-5829. PMID: 35701163, PMCID: PMC9337609, DOI: 10.1523/jneurosci.1385-21.2022.Peer-Reviewed Original ResearchConceptsSynaptotagmin-1C2 domainSynchronous neurotransmitter releaseSNARE proteinsPlasma membraneCalcium sensorSingle-pass transmembrane domainOptical tweezers measurementsSynaptic vesiclesAcidic lipidsNeurotransmitter releaseBind calciumCharge neutralization mutationsEvoked Neurotransmitter ReleaseReleasable vesicle poolSyt1 functionSNARE complexPolybasic regionOverall affinityTransmembrane domainMembrane bindingSingle-molecule experimentsC2A domainSynchronous releaseVesicle pool