Lipid-Dependent Bimodal MCL1 Membrane Activity
Landeta O, Valero J, Flores-Romero H, Bustillo-Zabalbeitia I, Landajuela A, Garcia-Porras M, Terrones O, Basañez G. Lipid-Dependent Bimodal MCL1 Membrane Activity. ACS Chemical Biology 2014, 9: 2852-2863. PMID: 25314294, DOI: 10.1021/cb500592e.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBcl-2-Associated X ProteinBH3 Interacting Domain Death Agonist ProteinCardiolipinsCholesterolFibroblastsGene ExpressionHeLa CellsHumansLiposomesMembrane LipidsMembrane Potential, MitochondrialMiceMitochondriaModels, MolecularMolecular Sequence DataMutagenesis, Site-DirectedMyeloid Cell Leukemia Sequence 1 ProteinPhosphatidylinositolsProtein Structure, TertiaryProto-Oncogene Proteins c-bcl-2Recombinant ProteinsSequence AlignmentConceptsPore-forming activityProtein functionConformational changesMitochondrial membrane lipidsLipid membrane environmentSite-directed mutagenesisProfound conformational changesNovel mechanistic insightsMembrane integrationCanonical BH3Homology regionMembrane environmentMembrane permeabilizationMitochondrial lipidsMinimal domainMembrane lipidsMitochondrial levelGroove interactionsProapoptotic partnersMcl1 activityMechanistic insightsMembrane activityModel membranesFluorescence spectroscopic analysisPermeabilization