2022
Membrane fission during bacterial spore development requires cellular inflation driven by DNA translocation
Landajuela A, Braun M, Martínez-Calvo A, Rodrigues CDA, Gomis Perez C, Doan T, Rudner DZ, Wingreen NS, Karatekin E. Membrane fission during bacterial spore development requires cellular inflation driven by DNA translocation. Current Biology 2022, 32: 4186-4200.e8. PMID: 36041438, PMCID: PMC9730832, DOI: 10.1016/j.cub.2022.08.014.Peer-Reviewed Original ResearchConceptsMother cell membraneMembrane fissionMother cellsDNA translocationMembrane tensionLarger mother cellMother cell cytoplasmCell membraneHigh membrane tensionSmaller foresporeDNA translocaseEnergy-limited conditionsForespore compartmentAsymmetric divisionEndospore formationMembrane compartmentsMembrane necksSpore developmentCell divisionMolecular basisForesporeMembrane flowBacillus subtilisCell cytoplasmFisB
2021
FisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria
Landajuela A, Braun M, Rodrigues CDA, Martínez-Calvo A, Doan T, Horenkamp F, Andronicos A, Shteyn V, Williams ND, Lin C, Wingreen NS, Rudner DZ, Karatekin E. FisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria. PLOS Biology 2021, 19: e3001314. PMID: 34185788, PMCID: PMC8274934, DOI: 10.1371/journal.pbio.3001314.Peer-Reviewed Original ResearchConceptsMembrane fissionMembrane fission machineryMother cell membraneNegative-curvature lipidsMother cell cytoplasmUnique membrane topologyFission machineryMembrane scissionMembrane topologyLipid microdomainsMembrane necksFission proteinsLipid bindingMembrane curvatureFilamentous cellsBacillus subtilisFisBProtein BCell cytoplasmAcidic lipidsCell membraneCurvature sensingImmobile clustersIntrinsic abilityBacteria