2022
Polybasic Patches in Both C2 Domains of Synaptotagmin-1 Are Required for Evoked Neurotransmitter Release
Wu Z, Ma L, Courtney NA, Zhu J, Landajuela A, Zhang Y, Chapman ER, Karatekin E. Polybasic Patches in Both C2 Domains of Synaptotagmin-1 Are Required for Evoked Neurotransmitter Release. Journal Of Neuroscience 2022, 42: 5816-5829. PMID: 35701163, PMCID: PMC9337609, DOI: 10.1523/jneurosci.1385-21.2022.Peer-Reviewed Original ResearchConceptsSynaptotagmin-1C2 domainSynchronous neurotransmitter releaseSNARE proteinsPlasma membraneCalcium sensorSingle-pass transmembrane domainOptical tweezers measurementsSynaptic vesiclesAcidic lipidsNeurotransmitter releaseBind calciumCharge neutralization mutationsEvoked Neurotransmitter ReleaseReleasable vesicle poolSyt1 functionSNARE complexPolybasic regionOverall affinityTransmembrane domainMembrane bindingSingle-molecule experimentsC2A domainSynchronous releaseVesicle pool
2021
FisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria
Landajuela A, Braun M, Rodrigues CDA, Martínez-Calvo A, Doan T, Horenkamp F, Andronicos A, Shteyn V, Williams ND, Lin C, Wingreen NS, Rudner DZ, Karatekin E. FisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria. PLOS Biology 2021, 19: e3001314. PMID: 34185788, PMCID: PMC8274934, DOI: 10.1371/journal.pbio.3001314.Peer-Reviewed Original ResearchConceptsMembrane fissionMembrane fission machineryMother cell membraneNegative-curvature lipidsMother cell cytoplasmUnique membrane topologyFission machineryMembrane scissionMembrane topologyLipid microdomainsMembrane necksFission proteinsLipid bindingMembrane curvatureFilamentous cellsBacillus subtilisFisBProtein BCell cytoplasmAcidic lipidsCell membraneCurvature sensingImmobile clustersIntrinsic abilityBacteria