2022
Structure-guided insights into potential function of novel genetic variants in the malaria vaccine candidate PfRh5
Mangou K, Moore A, Thiam L, Ba A, Orfanó A, Desamours I, Ndegwa D, Goodwin J, Guo Y, Sheng Z, Patel S, Diallo F, Sene S, Pouye M, Faye A, Thiam A, Nunez V, Diagne C, Sadio B, Shapiro L, Faye O, Mbengue A, Bei A. Structure-guided insights into potential function of novel genetic variants in the malaria vaccine candidate PfRh5. Scientific Reports 2022, 12: 19403. PMID: 36371450, PMCID: PMC9653458, DOI: 10.1038/s41598-022-23929-9.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, ProtozoanAntigens, ProtozoanCarrier ProteinsHumansMalaria VaccinesMalaria, FalciparumPlasmodium falciparumProtozoan ProteinsConceptsImmune evasionSingle nucleotide polymorphismsPopulation prevalenceVaccine-induced protective immunityP. falciparum positive samplesFalciparum positive samplesPlasmodium falciparum antigensMalaria vaccine candidateNovel single nucleotide polymorphismsInhibitory monoclonal antibodiesProtective immunityFalciparum antigensMalaria deathsEffective vaccineEfficacious vaccineVaccine candidatesPfRH5Infected individualsVaccine targetsMonoclonal antibodiesLow overall frequencyReceptor bindingNovel genetic variantsVaccineOverall frequency
2014
Immune Characterization of Plasmodium falciparum Parasites with a Shared Genetic Signature in a Region of Decreasing Transmission
Bei AK, Diouf A, Miura K, Larremore DB, Ribacke U, Tullo G, Moss EL, Neafsey DE, Daniels RF, Zeituni AE, Nosamiefan I, Volkman SK, Ahouidi AD, Ndiaye D, Dieye T, Mboup S, Buckee CO, Long CA, Wirth DF. Immune Characterization of Plasmodium falciparum Parasites with a Shared Genetic Signature in a Region of Decreasing Transmission. Infection And Immunity 2014, 83: 276-285. PMID: 25368109, PMCID: PMC4288878, DOI: 10.1128/iai.01979-14.Peer-Reviewed Original ResearchConceptsQuantitative reverse transcription PCRCommon genetic signatureSurface antigenClinical isolatesVar genesPlasmodium falciparum parasite populationsVariant surface antigensPlasmodium falciparum parasitesParasite-infected erythrocytesImmune characterizationMajor variant surface antigenSpecific parasite genotypesReverse transcription-PCRImmune responseFalciparum parasitesImmune recognitionMalaria transmissionVar transcriptsInfected erythrocytesParasite growthParasite clonesTranscription-PCRAntibodiesParasite correlatesParasite genotypesSTEVOR Is a Plasmodium falciparum Erythrocyte Binding Protein that Mediates Merozoite Invasion and Rosetting
Niang M, Bei AK, Madnani KG, Pelly S, Dankwa S, Kanjee U, Gunalan K, Amaladoss A, Yeo KP, Bob NS, Malleret B, Duraisingh MT, Preiser PR. STEVOR Is a Plasmodium falciparum Erythrocyte Binding Protein that Mediates Merozoite Invasion and Rosetting. Cell Host & Microbe 2014, 16: 81-93. PMID: 25011110, PMCID: PMC4382205, DOI: 10.1016/j.chom.2014.06.004.Peer-Reviewed Original ResearchConceptsMerozoite invasionFalciparum erythrocyte membrane protein 1P. falciparum erythrocyte membrane protein 1Erythrocyte membrane protein 1Variant surface antigensErythrocyte binding proteinInfected erythrocyte surfaceErythrocyte-binding proteinsMembrane protein 1STEVOR expressionMalaria pathogenesisRed blood cell surfaceImmune evasionSurface antigenUninfected RBCsBlood cell surfaceGlycophorin CInfected RBCsErythrocyte surfaceParasite infectionProtein 1Invasive merozoitesForm rosettesSTEVORSchizont stageAnalysis of pfhrp2 genetic diversity in Senegal and implications for use of rapid diagnostic tests
Deme AB, Park DJ, Bei AK, Sarr O, Badiane AS, Gueye Pel H, Ahouidi A, Ndir O, Mboup S, Wirth DF, Ndiaye D, Volkman SK. Analysis of pfhrp2 genetic diversity in Senegal and implications for use of rapid diagnostic tests. Malaria Journal 2014, 13: 34. PMID: 24472178, PMCID: PMC3913323, DOI: 10.1186/1475-2875-13-34.Peer-Reviewed Original ResearchConceptsGenetic diversityNucleotide diversityNon-synonymous nucleotide diversityRepeat length polymorphismAmino acid repeatsPfhrp2 geneLength polymorphismExtent of polymorphismEssential genesParasite ecologyAcid repeatsDiversity surveysParasite proteinsBp regionGenesProtein 2Polymorphic genesRepeatsDiversityHistidine-rich protein 2PfHRP2 antigenSenegalese National Malaria Control ProgrammeHRP2 detectionAntigen diversityPlasmodium falciparum
2013
Plasmodium falciparum Merozoite Surface Antigen, PfRH5, Elicits Detectable Levels of Invasion-Inhibiting Antibodies in Humans
Patel SD, Ahouidi AD, Bei AK, Dieye TN, Mboup S, Harrison SC, Duraisingh MT. Plasmodium falciparum Merozoite Surface Antigen, PfRH5, Elicits Detectable Levels of Invasion-Inhibiting Antibodies in Humans. The Journal Of Infectious Diseases 2013, 208: 1679-1687. PMID: 23904294, PMCID: PMC3805239, DOI: 10.1093/infdis/jit385.Peer-Reviewed Original ResearchConceptsMerozoite surface antigenSurface antigenPlasmodium falciparum merozoite surface antigenMulti-subunit vaccineMillions of infectionsIntracellular protozoan parasiteInvasion inhibition assaysSubstantial morbidityClinical isolatesP. falciparumBlood stagesPlasmodium falciparumNatural infectionAntibodiesPfRH5Protozoan parasiteDetectable levelsInhibition assaysInfectionAntigenFalciparumRecombinant formMorbidityLaboratory strainsVaccineInhibitory Humoral Responses to the Plasmodium falciparum Vaccine Candidate EBA-175 Are Independent of the Erythrocyte Invasion Pathway
Badiane AS, Bei AK, Ahouidi AD, Patel SD, Salinas N, Ndiaye D, Sarr O, Ndir O, Tolia NH, Mboup S, Duraisingh MT. Inhibitory Humoral Responses to the Plasmodium falciparum Vaccine Candidate EBA-175 Are Independent of the Erythrocyte Invasion Pathway. MSphere 2013, 20: 1238-1245. PMID: 23761656, PMCID: PMC3754519, DOI: 10.1128/cvi.00135-13.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayEBA-175Human antibodiesAffinity-purified human antibodiesBlood-stage vaccine candidateParasite strainsSialic acid-dependent mannerParasite invasionPresence of antibodiesErythrocyte-binding domainMultiple ligand-receptor interactionsEx vivo assaysPooled patient plasmaSenegalese parasitesAlternative invasion pathwaysHumoral responseVaccine candidatesPatient plasmaSenegalese patientsLigand-receptor interactionsClinical isolatesInvasion inhibitionVaccine designEx vivoFunctional inhibition
2007
Variant merozoite protein expression is associated with erythrocyte invasion phenotypes in Plasmodium falciparum isolates from Tanzania
Bei AK, Membi CD, Rayner JC, Mubi M, Ngasala B, Sultan AA, Premji Z, Duraisingh MT. Variant merozoite protein expression is associated with erythrocyte invasion phenotypes in Plasmodium falciparum isolates from Tanzania. Molecular And Biochemical Parasitology 2007, 153: 66-71. PMID: 17303262, DOI: 10.1016/j.molbiopara.2007.01.007.Peer-Reviewed Original Research