2023
Identification of an in vitro artemisinin-resistant Plasmodium falciparum kelch13 R515K mutant parasite in Senegal
Sene S, Pouye M, Martins R, Diallo F, Mangou K, Bei A, Barry A, Faye O, Ndiaye O, Faye O, Sall A, Lopez-Rubio J, Mbengue A. Identification of an in vitro artemisinin-resistant Plasmodium falciparum kelch13 R515K mutant parasite in Senegal. Frontiers In Parasitology 2023, 2: 1076759. DOI: 10.3389/fpara.2023.1076759.Peer-Reviewed Original ResearchPlasmodium falciparum clinical isolatesRing-stage survival assayArtesunate-amodiaquine treatmentPlasmodium falciparum malaria parasitesSentinel surveillance systemFalciparum malaria parasitesK mutationDifferent healthcare centersWorldwide malaria burdenPersistent feverAntimalarial treatmentMalaria-infected blood samplesMalaria casesMalaria burdenIndex caseBlood samplesHealthcare centersClinical isolatesMalaria parasitesSurvival assaysMutant parasitesSurveillance systemFunctional significanceTreatmentParasites
2018
De Novo Mutations Resolve Disease Transmission Pathways in Clonal Malaria
Redmond SN, MacInnis BM, Bopp S, Bei AK, Ndiaye D, Hartl DL, Wirth DF, Volkman SK, Neafsey DE. De Novo Mutations Resolve Disease Transmission Pathways in Clonal Malaria. Molecular Biology And Evolution 2018, 35: 1678-1689. PMID: 29722884, PMCID: PMC5995194, DOI: 10.1093/molbev/msy059.Peer-Reviewed Original ResearchConceptsDe novo mutationsEvolutionary ratesSlow evolutionary rateNovo mutationsComplex life cycleSlow generation timeLow-complexity regionsGenomic regionsLarge genomesGenomic epidemiology approachReintroduction scenariosCombination of sequencingP. falciparumViral speciesMutation rateClonal lineagesGenomeMutation studiesLibrary preparationIdentical parasitesGeneration timeBacterial pathogensMalaria parasitesMutationsGenomic epidemiology
2016
Ancient human sialic acid variant restricts an emerging zoonotic malaria parasite
Dankwa S, Lim C, Bei AK, Jiang RH, Abshire JR, Patel SD, Goldberg JM, Moreno Y, Kono M, Niles JC, Duraisingh MT. Ancient human sialic acid variant restricts an emerging zoonotic malaria parasite. Nature Communications 2016, 7: 11187. PMID: 27041489, PMCID: PMC4822025, DOI: 10.1038/ncomms11187.Peer-Reviewed Original ResearchConceptsInvasion ligandsSialic acid-independent pathwaysHuman RBCsZoonotic malaria parasiteAbsence of Neu5GcPlasmodium knowlesiMalaria parasitesCell surface receptorsN-glycolylneuraminic acidZoonotic parasitesSialic acid variantsSurface receptorsRBCsReceptorsNeu5GcMacaquesInvasionParasitesCMAHAcetylneuraminic acidAcid variantsHumansMalariaKnowlesi
2013
Expansion of host cellular niche can drive adaptation of a zoonotic malaria parasite to humans
Lim C, Hansen E, DeSimone TM, Moreno Y, Junker K, Bei A, Brugnara C, Buckee CO, Duraisingh MT. Expansion of host cellular niche can drive adaptation of a zoonotic malaria parasite to humans. Nature Communications 2013, 4: 1638. PMID: 23535659, PMCID: PMC3762474, DOI: 10.1038/ncomms2612.Peer-Reviewed Original ResearchConceptsRed blood cellsParasite densityP. knowlesiHuman bloodPlasmodium falciparum infectionMalaria parasite Plasmodium knowlesiYoung red blood cellsZoonotic malaria parasiteHigh parasite densityOld red blood cellsFalciparum infectionClinical infectionSevere diseaseWide age rangeNormal human bloodPlasmodium knowlesiMalaria parasitesBlood cellsImportant zoonosisAge rangeInfectionBloodKnowlesiYoung cellsCulture system
2012
SNP Genotyping Identifies New Signatures of Selection in a Deep Sample of West African Plasmodium falciparum Malaria Parasites
Amambua-Ngwa A, Park DJ, Volkman SK, Barnes KG, Bei AK, Lukens AK, Sene P, Van Tyne D, Ndiaye D, Wirth DF, Conway DJ, Neafsey DE, Schaffner SF. SNP Genotyping Identifies New Signatures of Selection in a Deep Sample of West African Plasmodium falciparum Malaria Parasites. Molecular Biology And Evolution 2012, 29: 3249-3253. PMID: 22688945, PMCID: PMC3472499, DOI: 10.1093/molbev/mss151.Peer-Reviewed Original ResearchConceptsHigh-density single nucleotide polymorphism arraysRecent selective sweepLocal selection pressuresSignals of selectionDrug resistance lociSingle nucleotide polymorphism arrayHaplotype-based testsPlasmodium falciparum malaria parasitesSelective sweepsResistance lociGenetic diversityGenomic regionsRecent selectionGenomic analysisSelection signalsPositive selectionSelection pressureFalciparum malaria parasitesPolymorphism arrayAntigenic lociMalaria parasitesMosquito vectorsEndemic populationsLociParasites
2007
Molecular Analysis of Erythrocyte Invasion in Plasmodium falciparum Isolates from Senegal
Jennings CV, Ahouidi AD, Zilversmit M, Bei AK, Rayner J, Sarr O, Ndir O, Wirth DF, Mboup S, Duraisingh MT. Molecular Analysis of Erythrocyte Invasion in Plasmodium falciparum Isolates from Senegal. Infection And Immunity 2007, 75: 3531-3538. PMID: 17470537, PMCID: PMC1932937, DOI: 10.1128/iai.00122-07.Peer-Reviewed Original ResearchConceptsInvasion pathwaysSequence polymorphismsMolecular analysisDistinct invasion pathwaysC-terminal regionMalaria parasitesSpecific sequence variantsGene amplificationHuman malaria parasiteLigand-receptor interactionsExpression polymorphismsRepeat regionProtein expression levelsSequence deletionsHomolog familyMultiple ligand-receptor interactionsLarge sequence deletionsSequence variantsSignificant sequence polymorphismErythrocyte invasionErythrocyte receptorP. falciparum parasitesExpression levelsPfRh1Low transmission areas