2024
Polarized localization of kinesin-1 and RIC-7 drives axonal mitochondria anterograde transport
Wu Y, Ding C, Sharif B, Weinreb A, Swaim G, Hao H, Yogev S, Watanabe S, Hammarlund M. Polarized localization of kinesin-1 and RIC-7 drives axonal mitochondria anterograde transport. Journal Of Cell Biology 2024, 223: e202305105. PMID: 38470363, PMCID: PMC10932739, DOI: 10.1083/jcb.202305105.Peer-Reviewed Original ResearchConceptsKinesin-1C. elegansN-terminal domainRetrograde trafficAnterograde trafficTransport mitochondriaMitochondria transportPolar localizationMiro-1CRISPR engineeringMitochondria localizationDisordered regionsMitochondriaTransport complexMitochondria distributionAxonal transportAnterograde transportAnterograde axonal transportMotor complexMiroAdaptorCRISPRGenes
2023
The neuropeptidergic connectome of C. elegans
Ripoll-Sánchez L, Watteyne J, Sun H, Fernandez R, Taylor S, Weinreb A, Bentley B, Hammarlund M, Miller D, Hobert O, Beets I, Vértes P, Schafer W. The neuropeptidergic connectome of C. elegans. Neuron 2023, 111: 3570-3589.e5. PMID: 37935195, PMCID: PMC7615469, DOI: 10.1016/j.neuron.2023.09.043.Peer-Reviewed Original ResearchConceptsNervous systemSynaptic wiring diagramGene expression datasetsReceptor-ligand interactionsStudied neuronsKey network hubNeuronal connectionsSignaling cascadesBrain functionInput connectivityNeuromodulatory signalingChemical synapsesPeptidergic neuromodulationBiochemical analysisEssential roleNeural basisNeuropeptidesConnectomeNetwork hubsWiring diagramSimilar pattern
2021
Molecular topography of an entire nervous system
Taylor SR, Santpere G, Weinreb A, Barrett A, Reilly MB, Xu C, Varol E, Oikonomou P, Glenwinkel L, McWhirter R, Poff A, Basavaraju M, Rafi I, Yemini E, Cook SJ, Abrams A, Vidal B, Cros C, Tavazoie S, Sestan N, Hammarlund M, Hobert O, Miller DM. Molecular topography of an entire nervous system. Cell 2021, 184: 4329-4347.e23. PMID: 34237253, PMCID: PMC8710130, DOI: 10.1016/j.cell.2021.06.023.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsFluorescent DyesGene Expression Regulation, DevelopmentalGenes, ReporterLarvaNervous SystemNeuronsNeuropeptidesNucleotide MotifsRegulatory Sequences, Nucleic AcidRNA-SeqSignal TransductionTranscription FactorsTranscription, GeneticConceptsGene expressionSpecific gene familiesCis-regulatory elementsNeuron-specific gene expressionIndividual neuron classesSingle-cell resolutionGene expression profilesNeuron classesGene familyAdhesion proteinsNeuropeptide genesExpression profilesExpression dataEntire nervous systemCombinatorial expressionMolecular topographyNervous systemSynaptic specificityNeuropeptide receptorsExpressionPotential roleWiring diagramComputational approachGenesProteinA head-to-head comparison of ribodepletion and polyA selection approaches for Caenorhabditis elegans low input RNA-sequencing libraries
Barrett A, McWhirter R, Taylor SR, Weinreb A, Miller DM, Hammarlund M. A head-to-head comparison of ribodepletion and polyA selection approaches for Caenorhabditis elegans low input RNA-sequencing libraries. G3: Genes, Genomes, Genetics 2021, 11: jkab121. PMID: 33856427, PMCID: PMC8495925, DOI: 10.1093/g3journal/jkab121.Peer-Reviewed Original ResearchConceptsC. elegans RNANoisy gene expressionRRNA gene sequencesRNA sequencing librariesRare cell populationsSuch challenging samplesCaenorhabditis elegansLong genesRibosomal RNAGene sequencesNoncoding RNAsCustom kitRNAseq librariesGene expressionPolyA selectionRRNA depletionLibrary preparationLibrary constructionRNAPCR duplicatesGenesRibodepletionCell populationsCell samplesExpression