Molecular topography of an entire nervous system
Taylor SR, Santpere G, Weinreb A, Barrett A, Reilly MB, Xu C, Varol E, Oikonomou P, Glenwinkel L, McWhirter R, Poff A, Basavaraju M, Rafi I, Yemini E, Cook SJ, Abrams A, Vidal B, Cros C, Tavazoie S, Sestan N, Hammarlund M, Hobert O, Miller DM. Molecular topography of an entire nervous system. Cell 2021, 184: 4329-4347.e23. PMID: 34237253, PMCID: PMC8710130, DOI: 10.1016/j.cell.2021.06.023.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsFluorescent DyesGene Expression Regulation, DevelopmentalGenes, ReporterLarvaNervous SystemNeuronsNeuropeptidesNucleotide MotifsRegulatory Sequences, Nucleic AcidRNA-SeqSignal TransductionTranscription FactorsTranscription, GeneticConceptsGene expressionSpecific gene familiesCis-regulatory elementsNeuron-specific gene expressionIndividual neuron classesSingle-cell resolutionGene expression profilesNeuron classesGene familyAdhesion proteinsNeuropeptide genesExpression profilesExpression dataEntire nervous systemCombinatorial expressionMolecular topographyNervous systemSynaptic specificityNeuropeptide receptorsExpressionPotential roleWiring diagramComputational approachGenesProteinA head-to-head comparison of ribodepletion and polyA selection approaches for Caenorhabditis elegans low input RNA-sequencing libraries
Barrett A, McWhirter R, Taylor SR, Weinreb A, Miller DM, Hammarlund M. A head-to-head comparison of ribodepletion and polyA selection approaches for Caenorhabditis elegans low input RNA-sequencing libraries. G3: Genes, Genomes, Genetics 2021, 11: jkab121. PMID: 33856427, PMCID: PMC8495925, DOI: 10.1093/g3journal/jkab121.Peer-Reviewed Original ResearchConceptsC. elegans RNANoisy gene expressionRRNA gene sequencesRNA sequencing librariesRare cell populationsSuch challenging samplesCaenorhabditis elegansLong genesRibosomal RNAGene sequencesNoncoding RNAsCustom kitRNAseq librariesGene expressionPolyA selectionRRNA depletionLibrary preparationLibrary constructionRNAPCR duplicatesGenesRibodepletionCell populationsCell samplesExpression