2011
Uterine and ovarian carcinosarcomas overexpressing Trop-2 are sensitive to hRS7, a humanized anti-Trop-2 antibody
Raji R, Guzzo F, Carrara L, Varughese J, Cocco E, Bellone S, Betti M, Todeschini P, Gasparrini S, Ratner E, Silasi DA, Azodi M, Schwartz P, Rutherford TJ, Buza N, Pecorelli S, Santin AD. Uterine and ovarian carcinosarcomas overexpressing Trop-2 are sensitive to hRS7, a humanized anti-Trop-2 antibody. Journal Of Experimental & Clinical Cancer Research 2011, 30: 106. PMID: 22075385, PMCID: PMC3224774, DOI: 10.1186/1756-9966-30-106.Peer-Reviewed Original ResearchConceptsAntibody-dependent cellular cytotoxicityAnti-Trop-2 antibodyTrop-2Cell linesEffective treatment optionChromium release assaysComplement-dependent cytotoxicityCarcinosarcoma cell lineCell surface markersOvarian carcinosarcomaTreatment optionsControl antibodyHRS7Cellular cytotoxicityHigher positivityTherapeutic strategiesHuman uterineTumor tissueFlow cytometryImmunohistochemistryRT-PCRSurface expressionAntibodiesHuman IgGCarcinosarcoma
1999
Chromosomal latency and expression at map unit 96 of a wild-type plus adeno-associated virus (AAV)/Neo vector and identification of p81, a new AAV transcriptional promoter.
Hermonat PL, Santin AD, De Greve J, De Rijcke M, Bishop BM, Han L, Mane M, Kokorina N. Chromosomal latency and expression at map unit 96 of a wild-type plus adeno-associated virus (AAV)/Neo vector and identification of p81, a new AAV transcriptional promoter. Human Virology 1999, 2: 359-68. PMID: 10774553.Peer-Reviewed Original Research
1998
Involvement of protein-DNA interaction in adeno-associated virus Rep78-mediated inhibition of HIV-1.
Kokorina NA, Santin AD, Li C, Hermonat PL. Involvement of protein-DNA interaction in adeno-associated virus Rep78-mediated inhibition of HIV-1. Human Virology 1998, 1: 441-50. PMID: 10195265.Peer-Reviewed Original ResearchConceptsProtein-DNA interactionsDNA binding abilityGene expressionHIV-1 long terminal repeat (LTR) sequencesTATA box regionLong terminal repeat sequencesHIV-LTR DNATerminal repeat sequencesRep78 proteinTranscription initiationTranscription factorsTATA boxRepeat sequencesVirus Rep78Rep78Box regionAmino acidsDNAInhibition of TatLikely targetMechanism of inhibitionMutantsHIV LTRElectrophoretic mobilityBindingThe effects of irradiation on the expression of a tumour rejection antigen (heat shock protein gp96) in human cervical cancer
Santin AD, Hermonat PL, Ravaggi A, Chiriva-Internati M, Hiserodt JC, Batchu RB, Pecorelli S, Parham GP. The effects of irradiation on the expression of a tumour rejection antigen (heat shock protein gp96) in human cervical cancer. International Journal Of Radiation Biology 1998, 73: 699-704. PMID: 9690688, DOI: 10.1080/095530098141951.Peer-Reviewed Original ResearchConceptsTumor rejection antigensHeat shock protein gp96Shock protein gp96Human cervical cancerCervical carcinoma cell linesRejection antigensHigh dosesHuman cervical carcinoma cell lineCervical cancerCarcinoma cell linesTumor cellsMajor histocompatibility complex class ILocal radiation therapyHistocompatibility complex class IBiologic response modifiersCo-stimulation moleculesComplex class ICell linesCervical cancer cellsDose-dependent mannerViral antigensResponse modifiersICAM-1Radiation therapyGp96 mRNA
1996
The adeno-associated virus Rep78 major regulatory/transformation suppressor protein binds cellular Sp1 in vitro and evidence of a biological effect.
Hermonat PL, Santin AD, Batchu RB. The adeno-associated virus Rep78 major regulatory/transformation suppressor protein binds cellular Sp1 in vitro and evidence of a biological effect. Cancer Research 1996, 56: 5299-304. PMID: 8912872.Peer-Reviewed Original ResearchConceptsCellular Sp1Cellular transcription factor Sp1Transformation suppressor proteinRNA polymerase IITranscription factor Sp1Transcription initiation processHuman chromosome 19Sp1-dependent promotersLong terminal repeat promoterSite-specific integrationAAV DNA replicationFactor Sp1Polymerase IISp1 DNAPossible downstream effectsDNA replicationCoimmunoprecipitation analysisMultifunctional proteinSuppressor proteinBiological effectsAmino halfHeterologous promoterChromosome 19H-RasTranscriptional activity