2011
Vascular Endothelial Growth Factor Receptor 2 Direct Interaction with Nephrin Links VEGF-A Signals to Actin in Kidney Podocytes*
Bertuccio C, Veron D, Aggarwal PK, Holzman L, Tufro A. Vascular Endothelial Growth Factor Receptor 2 Direct Interaction with Nephrin Links VEGF-A Signals to Actin in Kidney Podocytes*. Journal Of Biological Chemistry 2011, 286: 39933-39944. PMID: 21937443, PMCID: PMC3220571, DOI: 10.1074/jbc.m111.241620.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdaptor Proteins, Signal TransducingAnimalsChlorocebus aethiopsCOS CellsMass SpectrometryMembrane ProteinsMiceMice, TransgenicMultiprotein ComplexesOncogene ProteinsPhosphorylationPodocytesProtein Structure, TertiarySignal TransductionVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2ConceptsVEGF receptor 2Cytoplasmic domainTransmembrane protein nephrinBlot overlay experimentsSpecialized cell junctionsFoot process structurePodocyte actin cytoskeletonExtracellular cuesActin cytoskeletonGrowth factorMultiprotein interactionsTyrosine phosphorylationOverlay experimentsPodocyte cytoskeletonPodocyte foot processesCell junctionsPodocyte shapeCultured cellsKidney podocytesProtein nephrinSlit diaphragmDirect interactionCytoskeletonEpithelial cellsCultured podocytesPodocyte vascular endothelial growth factor (Vegf164) overexpression causes severe nodular glomerulosclerosis in a mouse model of type 1 diabetes
Veron D, Bertuccio CA, Marlier A, Reidy K, Garcia AM, Jimenez J, Velazquez H, Kashgarian M, Moeckel GW, Tufro A. Podocyte vascular endothelial growth factor (Vegf164) overexpression causes severe nodular glomerulosclerosis in a mouse model of type 1 diabetes. Diabetologia 2011, 54: 1227-1241. PMID: 21318407, PMCID: PMC3397150, DOI: 10.1007/s00125-010-2034-z.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternChromatography, High Pressure LiquidCreatinineDiabetes Mellitus, Type 1Diabetic NephropathiesDisease Models, AnimalEnzyme-Linked Immunosorbent AssayImmunohistochemistryMiceMice, TransgenicMicroscopy, Electron, TransmissionPodocytesPolymerase Chain ReactionSemaphorin-3ATandem Mass SpectrometryVascular Endothelial Growth Factor AConceptsDiabetic nephropathyNodular glomerulosclerosisDiabetic glomerulopathyMouse modelMassive proteinuriaExcessive vascular endothelial growth factorTransgenic miceStreptozotocin-induced mouse modelVascular endothelial growth factor overexpressionGlomerular basement membrane thickeningAdvanced diabetic glomerulopathyControl diabetic miceOnset of diabetesBasement membrane thickeningVascular endothelial growth factorType 1 diabetesGrowth factor overexpressionAdult transgenic miceEndothelial growth factorVEGF receptor 2Kimmelstiel-WilsonSystemic VEGFDiabetic micePathogenic roleRenal morphology
2010
Induction of Podocyte VEGF164 Overexpression at Different Stages of Development Causes Congenital Nephrosis or Steroid-Resistant Nephrotic Syndrome
Veron D, Reidy K, Marlier A, Bertuccio C, Villegas G, Jimenez J, Kashgarian M, Tufro A. Induction of Podocyte VEGF164 Overexpression at Different Stages of Development Causes Congenital Nephrosis or Steroid-Resistant Nephrotic Syndrome. American Journal Of Pathology 2010, 177: 2225-2233. PMID: 20829436, PMCID: PMC2966782, DOI: 10.2353/ajpath.2010.091146.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsHumansKidneyMiceMice, TransgenicNephrosis, LipoidNephrotic SyndromePodocytesProteinuriaVascular Endothelial Growth Factor AConceptsNephrotic syndromePodocyte effacementTransgenic miceSteroid-resistant nephrotic syndromeEndothelial cellsSingle transgenic miceMultiple renal diseasesSwollen endothelial cellsCongenital nephrotic syndromeVascular endothelial growthInducible transgenic miceNormal endothelial cellsGlomerular filtration barrierRenal diseasePathogenic rolePodocyte lossMice expressMassive albuminuriaEndothelial growthCongenital nephrosisMinimal changesFoot processesMiceGlomerulomegalyAlbuminuriaOverexpression of VEGF-A in podocytes of adult mice causes glomerular disease
Veron D, Reidy KJ, Bertuccio C, Teichman J, Villegas G, Jimenez J, Shen W, Kopp JB, Thomas DB, Tufro A. Overexpression of VEGF-A in podocytes of adult mice causes glomerular disease. Kidney International 2010, 77: 989-999. PMID: 20375978, DOI: 10.1038/ki.2010.64.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnimalsAutocrine CommunicationDiabetic NephropathiesGenotypeGlomerular Basement MembraneKidney DiseasesMatrix Metalloproteinase 9Membrane ProteinsMiceMice, TransgenicParacrine CommunicationPhenotypePhosphorylationPodocytesProtein BindingProteinuriaSignal TransductionUp-RegulationVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2ConceptsGlomerular diseaseAdult miceGlomerular basement membrane thickeningMurine diabetic nephropathyBasement membrane thickeningGlomerular endothelial cellsAdult transgenic miceOverexpression of VEGFExcessive VEGFDiabetic nephropathyGlomerular filtration barrierMesangial expansionPathogenic roleMetalloproteinase-9Functional abnormalitiesMembrane thickeningPodocyte effacementNephrin expressionReceptor 2Transgenic miceWhole kidneyGlomerular phenotypeEndothelial cellsParacrine VEGFVEGF
2009
Semaphorin3a regulates endothelial cell number and podocyte differentiation during glomerular development
Reidy KJ, Villegas G, Teichman J, Veron D, Shen W, Jimenez J, Thomas D, Tufro A. Semaphorin3a regulates endothelial cell number and podocyte differentiation during glomerular development. Development 2009, 136: 3979-3989. PMID: 19906865, PMCID: PMC2778745, DOI: 10.1242/dev.037267.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisCell CountCell DifferentiationCell NucleusCoculture TechniquesEndothelial CellsFluorescent Antibody Technique, DirectFluorescent DyesGene DeletionGene Expression Regulation, DevelopmentalGriffoniaImmunohistochemistryIn Situ Nick-End LabelingIndolesKidney GlomerulusMiceMice, KnockoutMice, Mutant StrainsMice, TransgenicPlant LectinsPodocytesRatsRecombinant ProteinsSemaphorin-3AConceptsVascular patterningPodocyte differentiationNegative regulatorNormal glomerular filtration barrierGlomerular developmentEssential negative regulatorEndothelial cell survivalFunction mouse modelsTight regulationEndothelial cell apoptosisUreteric budMature podocytesVascular developmentCell survivalCrucial roleGuidance proteinsSlit diaphragmCell apoptosisGlomerular filtration barrierRegulatorFiltration barrierGlomerular endothelial cell apoptosisCell numberEndothelial cellsEndothelial cell number