2013
Transport assays in filamentous fungi: Kinetic characterization of the UapC purine transporter of Aspergillus nidulans
Krypotou E, Diallinas G. Transport assays in filamentous fungi: Kinetic characterization of the UapC purine transporter of Aspergillus nidulans. Fungal Genetics And Biology 2013, 63: 1-8. PMID: 24355588, DOI: 10.1016/j.fgb.2013.12.004.Peer-Reviewed Original ResearchConceptsFilamentous ascomycete Aspergillus nidulansUric acid-xanthine transporterSpecificity profileAscomycete Aspergillus nidulansTransport assaysNucleobase-ascorbate transporter familySubstrate specificity profileMeasured apparent KmGenetic null mutantsAspergillus nidulansA. nidulansNull mutantsFilamentous fungiGerminating conidiosporesTransporter familyKinetic characterizationGenetic backgroundCompetition assaysVmax valuesMaximal expressionApparent KmUapCMorphological stagesAssayKinetic analysis
2012
Modeling, Substrate Docking, and Mutational Analysis Identify Residues Essential for the Function and Specificity of a Eukaryotic Purine-Cytosine NCS1 Transporter*
Krypotou E, Kosti V, Amillis S, Myrianthopoulos V, Mikros E, Diallinas G. Modeling, Substrate Docking, and Mutational Analysis Identify Residues Essential for the Function and Specificity of a Eukaryotic Purine-Cytosine NCS1 Transporter*. Journal Of Biological Chemistry 2012, 287: 36792-36803. PMID: 22969088, PMCID: PMC3481282, DOI: 10.1074/jbc.m112.400382.Peer-Reviewed Original ResearchMeSH KeywordsAdenineAmino Acid MotifsAmino Acid SubstitutionAspergillus nidulansConserved SequenceCytosineFungal ProteinsGuanineHydrogen BondingHypoxanthineKineticsMolecular Docking SimulationMolecular Dynamics SimulationMutagenesis, Site-DirectedPhenotypeProtein BindingProtein Structure, TertiarySequence Homology, Amino AcidStructural Homology, ProteinSubstrate SpecificitySymportersConceptsAsn-350Substrate binding siteAsn-163Substrate bindingSubstrate dockingSer-85Trp-259Substrate binding residuesPrimary sequence alignmentBinding sitesSubstrate binding affinitySystematic functional analysisTransmembrane a-helicesEukaryotic membersSequence alignmentBacterial membersA-helicesCritical residuesC-tailMicrobacterium liquefaciensRelevant mutantsCytoplasmic N-Binding residuesTMS1TMS6