Modeling, Substrate Docking, and Mutational Analysis Identify Residues Essential for the Function and Specificity of a Eukaryotic Purine-Cytosine NCS1 Transporter*
Krypotou E, Kosti V, Amillis S, Myrianthopoulos V, Mikros E, Diallinas G. Modeling, Substrate Docking, and Mutational Analysis Identify Residues Essential for the Function and Specificity of a Eukaryotic Purine-Cytosine NCS1 Transporter*. Journal Of Biological Chemistry 2012, 287: 36792-36803. PMID: 22969088, PMCID: PMC3481282, DOI: 10.1074/jbc.m112.400382.Peer-Reviewed Original ResearchMeSH KeywordsAdenineAmino Acid MotifsAmino Acid SubstitutionAspergillus nidulansConserved SequenceCytosineFungal ProteinsGuanineHydrogen BondingHypoxanthineKineticsMolecular Docking SimulationMolecular Dynamics SimulationMutagenesis, Site-DirectedPhenotypeProtein BindingProtein Structure, TertiarySequence Homology, Amino AcidStructural Homology, ProteinSubstrate SpecificitySymportersConceptsAsn-350Substrate binding siteAsn-163Substrate bindingSubstrate dockingSer-85Trp-259Substrate binding residuesPrimary sequence alignmentBinding sitesSubstrate binding affinitySystematic functional analysisTransmembrane a-helicesEukaryotic membersSequence alignmentBacterial membersA-helicesCritical residuesC-tailMicrobacterium liquefaciensRelevant mutantsCytoplasmic N-Binding residuesTMS1TMS6