2014
Modelling, substrate docking and mutational analysis identify residues essential for function and specificity of the major fungal purine transporter AzgA
Krypotou E, Lambrinidis G, Evangelidis T, Mikros E, Diallinas G. Modelling, substrate docking and mutational analysis identify residues essential for function and specificity of the major fungal purine transporter AzgA. Molecular Microbiology 2014, 93: 129-145. PMID: 24818808, DOI: 10.1111/mmi.12646.Peer-Reviewed Original ResearchConceptsSubstrate dockingMutation analysisNucleobase-ascorbate transporterAmino acid residuesNAT familyAspergillus nidulansInverted repeatsSubstrate bindingSubstrate translocationA-helicesPurine bindingC-tailAcid residuesTransporter familyCytoplasmic N-H(+) bindingAzgATMS8TMS10Founding memberFlexible domainsBinding cavityGate domainTMS3Residues
2012
Modeling, Substrate Docking, and Mutational Analysis Identify Residues Essential for the Function and Specificity of a Eukaryotic Purine-Cytosine NCS1 Transporter*
Krypotou E, Kosti V, Amillis S, Myrianthopoulos V, Mikros E, Diallinas G. Modeling, Substrate Docking, and Mutational Analysis Identify Residues Essential for the Function and Specificity of a Eukaryotic Purine-Cytosine NCS1 Transporter*. Journal Of Biological Chemistry 2012, 287: 36792-36803. PMID: 22969088, PMCID: PMC3481282, DOI: 10.1074/jbc.m112.400382.Peer-Reviewed Original ResearchMeSH KeywordsAdenineAmino Acid MotifsAmino Acid SubstitutionAspergillus nidulansConserved SequenceCytosineFungal ProteinsGuanineHydrogen BondingHypoxanthineKineticsMolecular Docking SimulationMolecular Dynamics SimulationMutagenesis, Site-DirectedPhenotypeProtein BindingProtein Structure, TertiarySequence Homology, Amino AcidStructural Homology, ProteinSubstrate SpecificitySymportersConceptsAsn-350Substrate binding siteAsn-163Substrate bindingSubstrate dockingSer-85Trp-259Substrate binding residuesPrimary sequence alignmentBinding sitesSubstrate binding affinitySystematic functional analysisTransmembrane a-helicesEukaryotic membersSequence alignmentBacterial membersA-helicesCritical residuesC-tailMicrobacterium liquefaciensRelevant mutantsCytoplasmic N-Binding residuesTMS1TMS6