2023
Decreased but diverse activity of cortical and thalamic neurons in consciousness-impairing rodent absence seizures
McCafferty C, Gruenbaum B, Tung R, Li J, Zheng X, Salvino P, Vincent P, Kratochvil Z, Ryu J, Khalaf A, Swift K, Akbari R, Islam W, Antwi P, Johnson E, Vitkovskiy P, Sampognaro J, Freedman I, Kundishora A, Depaulis A, David F, Crunelli V, Sanganahalli B, Herman P, Hyder F, Blumenfeld H. Decreased but diverse activity of cortical and thalamic neurons in consciousness-impairing rodent absence seizures. Nature Communications 2023, 14: 117. PMID: 36627270, PMCID: PMC9832004, DOI: 10.1038/s41467-022-35535-4.Peer-Reviewed Original ResearchConceptsThalamic neuronsAbsence seizuresSeizure onsetNeuronal firingConsciousness-impairing seizuresFemale rat modelLow-frequency electroencephalographic activityFunctional magnetic resonanceBehavioral arrestRat modelRhythmic firingTransient initial peakFiring increasesSeizuresNeuronal mechanismsElectroencephalographic activityNeuronsBrain state changesBrief episodesFiring decreasesInitial peakFiringMagnetic resonanceDistinct patternsOnset
2021
Small loci of astroglial glutamine synthetase deficiency in the postnatal brain cause epileptic seizures and impaired functional connectivity
Farina MG, Sandhu MRS, Parent M, Sanganahalli BG, Derbin M, Dhaher R, Wang H, Zaveri HP, Zhou Y, Danbolt NC, Hyder F, Eid T. Small loci of astroglial glutamine synthetase deficiency in the postnatal brain cause epileptic seizures and impaired functional connectivity. Epilepsia 2021, 62: 2858-2870. PMID: 34536233, PMCID: PMC9006438, DOI: 10.1111/epi.17072.Peer-Reviewed Original ResearchConceptsPostnatal brainFunctional connectivityContinuous video-electroencephalographic recordingSpontaneous recurrent seizuresPathogenesis of epilepsyGlutamine synthetase deficiencyVideo-electroencephalographic recordingsSpecific animal modelsEx vivo studySeizure thresholdAdult patientsRecurrent seizuresFocal epilepsyAdeno-associated virusHippocampal formationAnimal modelsFocal mannerEpileptic seizuresGS deficiencySmall lociSynthetase deficiencyEpilepsyGradual returnVivo studiesBrain
2015
Decreased Subcortical Cholinergic Arousal in Focal Seizures
Motelow JE, Li W, Zhan Q, Mishra AM, Sachdev RN, Liu G, Gummadavelli A, Zayyad Z, Lee HS, Chu V, Andrews JP, Englot DJ, Herman P, Sanganahalli BG, Hyder F, Blumenfeld H. Decreased Subcortical Cholinergic Arousal in Focal Seizures. Neuron 2015, 85: 561-572. PMID: 25654258, PMCID: PMC4319118, DOI: 10.1016/j.neuron.2014.12.058.Peer-Reviewed Original ResearchConceptsTemporal lobe seizuresFocal temporal lobe seizuresReduced cholinergic neurotransmissionBilateral temporal lobesLoss of consciousnessPedunculopontine tegmental nucleusQuality of lifeBrainstem arousal systemsFunctional magnetic resonanceSeizure spreadCholinergic neuronsImpaired consciousnessFocal seizuresBasal forebrainAnterior hypothalamusCholinergic neurotransmissionTegmental nucleusLateral septumCortical functionRodent modelsTemporal lobeSubcortical regionsSeizuresImpair consciousnessSubcortical arousalRhythmic 3–4Hz discharge is insufficient to produce cortical BOLD fMRI decreases in generalized seizures
Youngblood MW, Chen WC, Mishra AM, Enamandram S, Sanganahalli BG, Motelow JE, Bai HX, Frohlich F, Gribizis A, Lighten A, Hyder F, Blumenfeld H. Rhythmic 3–4Hz discharge is insufficient to produce cortical BOLD fMRI decreases in generalized seizures. NeuroImage 2015, 109: 368-377. PMID: 25562830, PMCID: PMC4340775, DOI: 10.1016/j.neuroimage.2014.12.066.Peer-Reviewed Original ResearchConceptsHz spike-wave dischargesSpike-wave dischargesFMRI decreasesSpike-wave seizuresAnimal modelsHuman patientsMagnetic resonance imaging studyTonic-clonic seizuresBlood oxygen level-dependent (BOLD) signalResonance imaging studyFunctional magnetic resonance imaging studyHuman functional magnetic resonance imaging (fMRI) studiesGeneralized seizuresFerret modelCortical decreasesAbsence seizuresCortical developmentAnesthetic regimeSeizuresTransient episodesHuman seizuresImaging studiesPatientsBOLD fMRISimultaneous electroencephalography
2014
Decreased Resting Functional Connectivity after Traumatic Brain Injury in the Rat
Mishra AM, Bai X, Sanganahalli BG, Waxman SG, Shatillo O, Grohn O, Hyder F, Pitkänen A, Blumenfeld H. Decreased Resting Functional Connectivity after Traumatic Brain Injury in the Rat. PLOS ONE 2014, 9: e95280. PMID: 24748279, PMCID: PMC3991600, DOI: 10.1371/journal.pone.0095280.Peer-Reviewed Original ResearchConceptsTraumatic brain injuryContralateral parietal cortexSham-operated animalsFunctional magnetic resonance imagingParietal cortexFunctional connectivityFunctional imagingBrain injuryAdult male Sprague-Dawley ratsLateral fluid percussion injuryExperimental traumatic brain injuryMale Sprague-Dawley ratsFluid percussion injuryPost-traumatic epileptogenesisSeizure-susceptibility testState BOLD-fMRISide of injuryAbnormal functional connectivitySham-operated ratsSprague-Dawley ratsMagnetic resonance imagingSeizure susceptibilitySkull electrodesEpileptiform dischargesFunctional imaging data