Jianfeng Lin, PhD
Cards
About
Titles
Senior Research Scientist
Managing Director, Yale CryoEM ResourceBiography
Dr. Jianfeng Lin obtained his PhD in Biochemistry and Molecular Biology from Xiamen University in 2008, and performed postdoctoral studies at Brandeis University and then at UT Southwestern Medical Center with Professor Daniela Nicastro. His research enhances the understanding of molecular mechanisms of ciliary motility (e.g. Lin and Nicastro, Science, 2018), dynein movement (e.g. Lin et al., Nat Cell Biol, 2014), and ciliopathies that lead to chronic respiratory disease (e.g. Lin et al., Nat Commun, 2014). In 2017, Dr. Lin joined Thermo Fisher Scientific as a Field Applications Scientist and supported the cryo-EM researchers in the U.S. and China. Dr. Lin started his appointment at the MB&B department as a Research Scientist in November 2020 to unify three EM facilities at Yale as a single functional unit, the Yale CryoEM Resource (YCR), and support both internal and external cryo-EM communities.
Appointments
Molecular Biophysics and Biochemistry
Senior Research ScientistPrimary
Other Departments & Organizations
Education & Training
- Postdoctoral Associate
- Brandeis University (2015)
- PhD
- Xiamen University, Biochemistry and Molecular Biology (2008)
- BS
- Xiamen University, Biological Sciences (2003)
Research
Publications
2019
FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia
Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O’Toole E, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME. FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Molecular Biology Of The Cell 2019, 30: 2659-2680. PMID: 31483737, PMCID: PMC6761771, DOI: 10.1091/mbc.e19-07-0367.Peer-Reviewed Original ResearchConceptsInner dynein armsRegulatory complexCryo-electron tomographyInner arm dyneinsCiliary motilityMultiple dynein motorsPrecise spatial organizationAxonemal repeatDocking factorUnique binding siteWD repeatsDynein assemblyAssembly factorsDomain proteinsRegulatory hubDynein complexDynein isoformsDynein subunitsInsertional mutagenesisNew lociRegulatory proteinsDynein motorsDifferent dyneinsDoublet microtubulesTransport factors
2018
Asymmetric distribution and spatial switching of dynein activity generates ciliary motility
Lin J, Nicastro D. Asymmetric distribution and spatial switching of dynein activity generates ciliary motility. Science 2018, 360 PMID: 29700238, PMCID: PMC6640125, DOI: 10.1126/science.aar1968.Peer-Reviewed Original ResearchConceptsDynein activitySea urchin sperm cellsCryo-electron tomographyIndividual dyneinsDynein isoformsAsymmetric distributionMotile ciliaDyneinFlagellaHypothesis positsSperm cellsSmall populationCiliary motilityFlagellar bendingCoordinated activityActive stateMotilityActivity stateOrganellesIsoformsCiliaActivity
2014
Cryo-electron tomography reveals ciliary defects underlying human RSPH1 primary ciliary dyskinesia
Lin J, Yin W, Smith MC, Song K, Leigh MW, Zariwala MA, Knowles MR, Ostrowski LE, Nicastro D. Cryo-electron tomography reveals ciliary defects underlying human RSPH1 primary ciliary dyskinesia. Nature Communications 2014, 5: 5727. PMID: 25473808, PMCID: PMC4267722, DOI: 10.1038/ncomms6727.Peer-Reviewed Original ResearchConceptsCryo-electron tomographyNative 3D structureNormal human developmentDistant speciesHuman ciliaCilia structurePCD phenotypeCilia dysfunctionHuman diseasesNative structurePrimary ciliary dyskinesiaRadial spokesCiliaEssential roleFunctional heterogeneityHuman respiratory ciliaUnprecedented detailPrimary defectCiliary dyskinesiaUnknown primary defectStructural mechanism of the dynein power stroke
Lin J, Okada K, Raytchev M, Smith MC, Nicastro D. Structural mechanism of the dynein power stroke. Nature Cell Biology 2014, 16: 479-485. PMID: 24727830, PMCID: PMC4102432, DOI: 10.1038/ncb2939.Peer-Reviewed Original Research
2012
One of the Nine Doublet Microtubules of Eukaryotic Flagella Exhibits Unique and Partially Conserved Structures
Lin J, Heuser T, Song K, Fu X, Nicastro D. One of the Nine Doublet Microtubules of Eukaryotic Flagella Exhibits Unique and Partially Conserved Structures. PLOS ONE 2012, 7: e46494. PMID: 23071579, PMCID: PMC3468612, DOI: 10.1371/journal.pone.0046494.Peer-Reviewed Original ResearchThe structural heterogeneity of radial spokes in cilia and flagella is conserved
Lin J, Heuser T, Carbajal‐González B, Song K, Nicastro D. The structural heterogeneity of radial spokes in cilia and flagella is conserved. Cytoskeleton 2012, 69: 88-100. PMID: 22170736, PMCID: PMC3307805, DOI: 10.1002/cm.21000.Peer-Reviewed Original ResearchConceptsFlagellar motilityRadial spokesNexin-dynein regulatory complexRegulatory complexFlagellar structureMost organismsThree-dimensional structureSubtomogram averagingCryoelectron tomographyDynein motorsDynein activityChlamydomonas flagellaMotile ciliaFlagellaSperm flagellaUbiquitous componentOrganismsFunctional differencesEssential roleCiliaRepeat unitsUnprecedented detailBasal partRS1Motility
2011
Building Blocks of the Nexin-Dynein Regulatory Complex in Chlamydomonas Flagella*
Lin J, Tritschler D, Song K, Barber CF, Cobb JS, Porter ME, Nicastro D. Building Blocks of the Nexin-Dynein Regulatory Complex in Chlamydomonas Flagella*. Journal Of Biological Chemistry 2011, 286: 29175-29191. PMID: 21700706, PMCID: PMC3190725, DOI: 10.1074/jbc.m111.241760.Peer-Reviewed Original ResearchConceptsNexin-dynein regulatory complexRegulatory complexImportant regulatory nodeTwo-dimensional electrophoresisRegulatory nodesProtein phosphorylationSignal transductionPhosphorylation statusMALDI-TOF mass spectrometryDynein motorsDynein activityChlamydomonas flagellaMotile ciliaRegulatory functionsPhosphorylated isoformsCoordination of thousandsFlagellar axonemeBiochemical comparisonRadial spokesPCR analysisFlagellar bendingIsoform patternFlagellaMolecular compositionProtein
2008
Stain efficiency and MALDI-TOF MS compatibility of seven visible staining procedures.
Lin JF, Chen QX, Tian HY, Gao X, Yu ML, Xu GJ, Zhao FK. Stain efficiency and MALDI-TOF MS compatibility of seven visible staining procedures. Analytical And Bioanalytical Chemistry 2008, 390: 1765-73. PMID: 18283439, DOI: 10.1007/s00216-008-1910-6.Peer-Reviewed Original Research
2007
Identification of candidate prostate cancer biomarkers in prostate needle biopsy specimens using proteomic analysis.
Lin JF, Xu J, Tian HY, Gao X, Chen QX, Gu Q, Xu GJ, Song JD, Zhao FK. Identification of candidate prostate cancer biomarkers in prostate needle biopsy specimens using proteomic analysis. International Journal Of Cancer. Journal International Du Cancer 2007, 121: 2596-605. PMID: 17722004, DOI: 10.1002/ijc.23016.Peer-Reviewed Original Research
2006
A novel droplet-tap sample-loading method for two-dimensional gel electrophoresis.
Long XH, Lin JF, Zhang YZ. A novel droplet-tap sample-loading method for two-dimensional gel electrophoresis. Anal Bioanal Chem 2006, 384: 1578-83. PMID: 16554959, DOI: 10.1007/s00216-006-0304-x.Peer-Reviewed Original Research