2018
Identification and functional study of the endoplasmic reticulum stress sensor IRE1 in Chlamydomonas reinhardtii
Yamaoka Y, Choi BY, Kim H, Shin S, Kim Y, Jang S, Song W, Cho CH, Yoon HS, Kohno K, Lee Y. Identification and functional study of the endoplasmic reticulum stress sensor IRE1 in Chlamydomonas reinhardtii. The Plant Journal 2018, 94: 91-104. PMID: 29385296, DOI: 10.1111/tpj.13844.Peer-Reviewed Original ResearchConceptsStress sensor IRE1Unfolded protein responseIre1 mutantsER stress responseMarker gene expressionGene expressionTandem zinc finger domainStress responseER stress sensor IRE1Cytosolic C-terminal regionEndoplasmic reticulum (ER) stress sensor IRE1Zinc finger domainER stress conditionsC-terminal regionN-terminal regionROS scavenger glutathioneER stress inducersStrain CCYeast Ire1pEvolutionary historyER homeostasisEndoplasmic reticulum stressChlamydomonas reinhardtiiProtein responseChimeric proteinEndoplasmic reticulum acyltransferase with prokaryotic substrate preference contributes to triacylglycerol assembly in Chlamydomonas
Kim Y, Terng EL, Riekhof WR, Cahoon EB, Cerutti H. Endoplasmic reticulum acyltransferase with prokaryotic substrate preference contributes to triacylglycerol assembly in Chlamydomonas. Proceedings Of The National Academy Of Sciences Of The United States Of America 2018, 115: 1652-1657. PMID: 29382746, PMCID: PMC5816170, DOI: 10.1073/pnas.1715922115.Peer-Reviewed Original ResearchMeSH KeywordsAcyltransferasesAlgal ProteinsChlamydomonas reinhardtiiChloroplastsEndoplasmic ReticulumPhylogenySubstrate SpecificityTriglyceridesConceptsUnicellular green algaEndoplasmic reticulum membraneLysophosphatidic acid acyltransferaseChloroplastic pathwayPrecursor biosynthesisTAG precursorsNitrogen deprivationTriacylglycerol assemblyAssembly pathwayGreen algaTAG accumulationAcid acyltransferaseAcyl donor substratesReticulum membraneBiomaterial productionDonor substrateChlamydomonasTriacylglycerol metabolismPalmitoyl-CoAAcyltransferasePathwayMajor roleBiosynthesisAlgaRNA
2017
A multidomain enzyme, with glycerol‐3‐phosphate dehydrogenase and phosphatase activities, is involved in a chloroplastic pathway for glycerol synthesis in Chlamydomonas reinhardtii
Morales‐Sánchez D, Kim Y, Terng EL, Peterson L, Cerutti H. A multidomain enzyme, with glycerol‐3‐phosphate dehydrogenase and phosphatase activities, is involved in a chloroplastic pathway for glycerol synthesis in Chlamydomonas reinhardtii. The Plant Journal 2017, 90: 1079-1092. PMID: 28273364, DOI: 10.1111/tpj.13530.Peer-Reviewed Original ResearchMeSH KeywordsChlamydomonas reinhardtiiChloroplastsGlycerolGlycerolphosphate DehydrogenasePhosphoric Monoester HydrolasesPlant ProteinsConceptsMultidomain enzymeNitrogen deprivationPhosphatase activityGreen alga C.Dihydroxyacetone phosphateGlycerol synthesisGlycerol-3-phosphate dehydrogenaseHigh salinityCore chlorophytesPhosphatase motifPlastid pathwayChloroplastic pathwayAlgal speciesDehydrogenase domainChlamydomonas reinhardtiiChlamydomonas cellsMembrane glycerolipidsHyperosmotic stressCompatible solutesTAG accumulationBifunctional enzymeAlgal metabolismHypertonic stressFusion proteinTriacylglycerol accumulation