NuMA Phosphorylation by Aurora-A Orchestrates Spindle Orientation
Gallini S, Carminati M, De Mattia F, Pirovano L, Martini E, Oldani A, Asteriti I, Guarguaglini G, Mapelli M. NuMA Phosphorylation by Aurora-A Orchestrates Spindle Orientation. Current Biology 2016, 26: 458-469. PMID: 26832443, DOI: 10.1016/j.cub.2015.12.051.Peer-Reviewed Original ResearchConceptsSpindle orientationSpindle polesPhosphorylation of NuMAMicrotubule motor dyneinMicrotubule-binding activityMicrotubule-binding domainNon-transformed cellsSpindle placementGαi complexesTissue morphogenesisCortical enrichmentDirect phosphorylationMotor dyneinSpindle positioningCell cortexSer residuesC-terminusFRAP experimentsDyneinPhosphorylationNuMAMicrotubulesTimely recruitmentDynamic exchangePartial inhibitionConcomitant binding of Afadin to LGN and F-actin directs planar spindle orientation
Carminati M, Gallini S, Pirovano L, Alfieri A, Bisi S, Mapelli M. Concomitant binding of Afadin to LGN and F-actin directs planar spindle orientation. Nature Structural & Molecular Biology 2016, 23: 155-163. PMID: 26751642, DOI: 10.1038/nsmb.3152.Peer-Reviewed Original Research