2018
Insc:LGN tetramers promote asymmetric divisions of mammary stem cells
Culurgioni S, Mari S, Bonetti P, Gallini S, Bonetto G, Brennich M, Round A, Nicassio F, Mapelli M. Insc:LGN tetramers promote asymmetric divisions of mammary stem cells. Nature Communications 2018, 9: 1025. PMID: 29523789, PMCID: PMC5844954, DOI: 10.1038/s41467-018-03343-4.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsAsymmetric Cell DivisionCell Cycle ProteinsCytoskeletal ProteinsDrosophilaDrosophila ProteinsGuanine Nucleotide Dissociation InhibitorsHumansIntracellular Signaling Peptides and ProteinsMembrane ProteinsMiceModels, MolecularProtein BindingStem CellsConceptsMammary stem cellsAsymmetric divisionStem cellsAsymmetric cell divisionStem cell proliferationProtein LGNDrosophila neuroblastsFate specificationFate determinantsTissue morphogenesisAsymmetric fatesCell divisionMicrotubule motorsSymmetric divisionTetrameric arrangementNovel roleAsymmetric domainsInscuteableCell proliferationINSCP53 lossStable coreDivisionCellsTetramer
2016
NuMA Phosphorylation by Aurora-A Orchestrates Spindle Orientation
Gallini S, Carminati M, De Mattia F, Pirovano L, Martini E, Oldani A, Asteriti I, Guarguaglini G, Mapelli M. NuMA Phosphorylation by Aurora-A Orchestrates Spindle Orientation. Current Biology 2016, 26: 458-469. PMID: 26832443, DOI: 10.1016/j.cub.2015.12.051.Peer-Reviewed Original ResearchConceptsSpindle orientationSpindle polesPhosphorylation of NuMAMicrotubule motor dyneinMicrotubule-binding activityMicrotubule-binding domainNon-transformed cellsSpindle placementGαi complexesTissue morphogenesisCortical enrichmentDirect phosphorylationMotor dyneinSpindle positioningCell cortexSer residuesC-terminusFRAP experimentsDyneinPhosphorylationNuMAMicrotubulesTimely recruitmentDynamic exchangePartial inhibition