Isolation and analysis of rare cells in the blood of cancer patients using a negative depletion methodology
Wu Y, Deighan C, Miller B, Balasubramanian P, Lustberg M, Zborowski M, Chalmers J. Isolation and analysis of rare cells in the blood of cancer patients using a negative depletion methodology. Methods 2013, 64: 169-182. PMID: 24056212, PMCID: PMC3874448, DOI: 10.1016/j.ymeth.2013.09.006.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkers, TumorBreast NeoplasmsCell CountCell Line, TumorFemaleFlow CytometryFluorescent Antibody TechniqueHumansImmunomagnetic SeparationLeukocyte Common AntigensNeoplastic Cells, CirculatingConceptsCancer patientsMetastatic breast cancer patientsBreast cancer patientsPeripheral blood samplesRed blood cell lysisRare cellsNon-hematopoietic cellsBlood cell lysisImmunomagnetic depletionCD45 antibodyBlood samplesLog depletionPatientsImmunofluorescence stainingNucleated cellsWhole bloodBloodCD45RBC lysisCellsCell lysisLysisAntibodies