2009
Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins
Stone K, Williams K. Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins. Springer Protocols Handbooks 2009, 941-950. DOI: 10.1007/978-1-59745-198-7_102.Peer-Reviewed Original ResearchLaser desorption mass spectrometrySites of chemicalHigh pressureUltra-high pressureRelative elution positionsBroad peakDesorption mass spectrometryPowerful techniquePhase resultsMobile phase resultsReversed-phase HPLCEnzymatic digestsLC systemRelevant parametersMatrix-assisted laser desorption mass spectrometryMass spectrometric approachReversed-phase HPLC separationReversephase HPLCTotal hydrophobicitySpectrometric approachAqueous mixturesMass spectrometryVolatile solventsResolutionHPLC separation
2002
Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins
Stone K, Williams K. Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins. 2002, 0: 533-540. DOI: 10.1385/1-59259-169-8:533.Peer-Reviewed Original ResearchReversed-phase HPLCEnzymatic digestsLaser desorption mass spectrometrySites of chemicalMatrix-assisted laser desorption mass spectrometryRelative elution positionsDesorption mass spectrometryMobile phase resultsReversed-phase HPLC separationTotal hydrophobicityAqueous mixturesMass spectrometryVolatile solventsReversephase HPLCHPLC separationLarge peptidesParticular separationComplex mixturesExcellent reproducibilitySlow kineticsEdman sequencingRetention coefficientTight bindingHydrophobic amino acidsHPLC
1996
Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins
Stone K, Williams K. Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins. Springer Protocols Handbooks 1996, 427-434. DOI: 10.1007/978-1-60327-259-9_72.Peer-Reviewed Original ResearchReversed-phase HPLCEnzymatic digestsLaser desorption mass spectrometrySites of chemicalMatrix-assisted laser desorption mass spectrometryRelative elution positionsDesorption mass spectrometryMobile phase resultsReversed-phase HPLC separationTotal hydrophobicityAqueous mixturesMass spectrometryVolatile solventsHPLC separationLarge peptidesComplex mixturesParticular separationExcellent reproducibilitySlow kineticsEdman sequencingRetention coefficientTight bindingHydrophobic amino acidsHPLCRelative insolubility