2009
Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels
Stone K, Gulcicek E, Williams K. Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels. Springer Protocols Handbooks 2009, 905-917. DOI: 10.1007/978-1-59745-198-7_99.Peer-Reviewed Original ResearchProtein of interestSDS-polyacrylamide gelsMass spectrometryGel digestion procedureChemical sequencingLaser desorption ionization mass spectrometryDesorption ionization mass spectrometryElectrospray ionization sourceIonization mass spectrometryComplex biological mixturesMass spectral acquisitionProtein identification analysisTandem mass spectrometrySDS-PAGEMass spectrometric analysisPolyacrylamide gelsIonization sourceNumerous genomesBiological mixturesPeptide mass dataProtein databaseCleavage procedureMass spectrometerGel matrixLC system
1996
Matrix-Assisted Laser Desorption Ionization Mass Spectrometry as a Complement to Internal Protein Sequencing
Williams K, Samandar S, Stone K, Saylor M, Rush J. Matrix-Assisted Laser Desorption Ionization Mass Spectrometry as a Complement to Internal Protein Sequencing. Springer Protocols Handbooks 1996, 541-555. DOI: 10.1007/978-1-60327-259-9_91.Peer-Reviewed Original ResearchLaser desorption ionization mass spectrometryDesorption ionization mass spectrometryIonization mass spectrometryMass spectrometryMatrix-Assisted Laser Desorption Ionization Mass SpectrometryInternal protein sequencingBiological moleculesMALDI-MSSimple instrumentationSpectrometryBiochemical laboratoryMass analysis methodProtein sequencingMoleculesHigh throughput