2002
Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels
Stone K, Williams K. Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels. 2002, 0: 511-521. DOI: 10.1385/1-59259-169-8:511.Peer-Reviewed Original ResearchPolyacrylamide gel matrixFree NH2 terminusPeptide fractionationCleavage procedureGel matrixFinal purificationChemical cleavagePolyacrylamide gelsEdman degradationMost eukaryotic proteinsSDS-polyacrylamide gelsPVDFGelSpecific proteasesPurificationSDS-PAGECleavageNH2 terminusFractionationPartial amino acid sequenceDegradation
1996
Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels
Stone K, Williams K. Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels. Springer Protocols Handbooks 1996, 415-425. DOI: 10.1007/978-1-60327-259-9_71.Peer-Reviewed Original ResearchGel digestion procedurePolyacrylamide gel matrixPolyacrylamide gelsFree NH2 terminusPeptide fractionationCleavage procedurePonceau SGel matrixFinal purificationReversed-phase HPLCSDS-polyacrylamide gelsChemical cleavageDigestion procedureCoomassie blueProteolytic digestionSitu digestionEnzymatic digestionGelEdman degradationNitrocellulose membraneMost eukaryotic proteinsSDS-PAGEPVDFSolutionDigestion
1992
Identification of amino acid residues at the interface of a bacteriophage T4 regA protein-nucleic acid complex.
Webster K, Keill S, Konigsberg W, Williams K, Spicer E. Identification of amino acid residues at the interface of a bacteriophage T4 regA protein-nucleic acid complex. Journal Of Biological Chemistry 1992, 267: 26097-26103. PMID: 1464621, DOI: 10.1016/s0021-9258(18)35722-3.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBacterial ProteinsBacteriophage T4Base SequenceBinding SitesChromatography, High Pressure LiquidCross-Linking ReagentsMolecular Sequence DataOligoribonucleotidesPeptide FragmentsPlasmidsPromoter Regions, GeneticRNA, MessengerRNA, ViralSequence Homology, Amino AcidTrypsinUltraviolet RaysConceptsCross-linked peptidesProtein-nucleic acid complexesAnion-exchange high-performance liquid chromatographyNucleic acidsIntact proteinHigh-performance liquid chromatographyCross-linked complexGas-phase sequencingPerformance liquid chromatographyAcid complexesExchange high performance liquid chromatographyLiquid chromatographyChemical cleavageBacteriophage T4 regA proteinNucleic acid bindingTryptic peptidesComplexesUltraviolet lightCNBr peptidesPeptidesCN6Amino acid residuesMeasurable affinityAcid bindingAcid
1990
[21] Reversed-phase high-performance liquid chromatography for fractionation of enzymatic digests and chemical cleavage products of proteins
Stone K, Elliott J, Peterson G, McMurray W, Williams K. [21] Reversed-phase high-performance liquid chromatography for fractionation of enzymatic digests and chemical cleavage products of proteins. Methods In Enzymology 1990, 193: 389-412. PMID: 2074828, DOI: 10.1016/0076-6879(90)93429-o.Peer-Reviewed Original ResearchConceptsHigh-performance liquid chromatographyReversed-phase high-performance liquid chromatographyReversed phase high performance liquid chromatographyLiquid chromatographyEnzymatic digestsHigh peak capacityMass spectrometric approachProtein chemistsSpectrometric approachMass spectrometryPeak capacityComplex mixturesMolecular weightChemical cleavageGradient timeCleavage productsChromatographyTryptic peptidesPeptidesDigestsChemistsSpectrometryFractionationProductsPrimary structure