1995
Digestion of Proteins in Gels for Sequence Analysis
Stone K, Williams K. Digestion of Proteins in Gels for Sequence Analysis. Current Protocols In Protein Science 1995, 00: 11.3.1-11.3.13. DOI: 10.1002/0471140864.ps1103s00.Peer-Reviewed Original ResearchProtein samplesSDS-PAGEDigestion of proteinsSDS-polyacrylamide gelsAbsence of detergentCDNA cloningAmino terminusPartial sequencesSequencing studiesSequence analysisInternal sequencingInhibits trypsinAmino acid analysisAmount of proteinProteinAlternate protocolGel slicesAcid analysisEnzymatic cleavageReversed-phase HPLCSubsequent washing stepsResidual SDSPmol amountsCloningTerminus
1991
State‐of‐the‐art biomolecular core facilities: a comprehensive survey1
Niece R, Beach C, Cook R, Hathaway G, Williams K. State‐of‐the‐art biomolecular core facilities: a comprehensive survey1. The FASEB Journal 1991, 5: 2756-2760. PMID: 1916100, DOI: 10.1096/fasebj.5.13.1916100.Peer-Reviewed Original ResearchAmino Acid Analysis and Sequencing — What is State-of-the-Art?
Niece R, Ericsson L, Fowler A, Smith A, Speicher D, Crabb J, Williams K. Amino Acid Analysis and Sequencing — What is State-of-the-Art? Advances In Life Sciences 1991, 133-141. DOI: 10.1007/978-3-0348-5678-2_12.Peer-Reviewed Original Research
1987
Ferrate oxidation of Escherichia coli DNA polymerase-I. Identification of a methionine residue that is essential for DNA binding.
Basu A, Williams K, Modak M. Ferrate oxidation of Escherichia coli DNA polymerase-I. Identification of a methionine residue that is essential for DNA binding. Journal Of Biological Chemistry 1987, 262: 9601-9607. PMID: 3298259, DOI: 10.1016/s0021-9258(18)47976-8.Peer-Reviewed Original Research
1982
Comparative Peptide Mapping by HPLC: Identification of Single Amino Acid Substitutions in Temperature Sensitive Mutants
Williams K, L’Italien J, Guggenheimer R, Sillerud L, Spicer E, Chase J, Konigsberg W. Comparative Peptide Mapping by HPLC: Identification of Single Amino Acid Substitutions in Temperature Sensitive Mutants. Experimental Biology And Medicine 1982, 499-507. DOI: 10.1007/978-1-4612-5832-2_44.Peer-Reviewed Original ResearchPeptide mappingChemical modificationCovalent proteinComparative peptide mappingAmino acid analysisProtein structureParticular amino acid replacementsPrimary structureLac repressor moleculesHuman hemoglobinHPLCAcid analysisSubstitutionStructurePowerful approachSingle amino acid substitutionCrosslinkingMoleculesAmino acid substitutionsSubtitutionAcid substitutionsMutant proteins