2015
SENP1-mediated NEMO deSUMOylation in adipocytes limits inflammatory responses and type-1 diabetes progression
Shao L, Zhou HJ, Zhang H, Qin L, Hwa J, Yun Z, Ji W, Min W. SENP1-mediated NEMO deSUMOylation in adipocytes limits inflammatory responses and type-1 diabetes progression. Nature Communications 2015, 6: 8917. PMID: 26596471, PMCID: PMC4662081, DOI: 10.1038/ncomms9917.Peer-Reviewed Original ResearchMeSH Keywords3T3-L1 CellsAdipocytesAnimalsApoptosisChemokine CCL5Chromatin ImmunoprecipitationCysteine EndopeptidasesCytokinesDiabetes Mellitus, Type 1Diabetes Mellitus, Type 2Diet, High-FatEndopeptidasesEnzyme-Linked Immunosorbent AssayFlow CytometryGene Knockout TechniquesGlucose IntoleranceHyperglycemiaImmunoblottingImmunoprecipitationInflammationInsulin ResistanceInsulin-Secreting CellsIntracellular Signaling Peptides and ProteinsIslets of LangerhansMiceMutagenesis, Site-DirectedNF-kappa BPhenotypeReverse Transcriptase Polymerase Chain ReactionSmall Ubiquitin-Related Modifier ProteinsConceptsNF-κB activityAdipocyte dysfunctionCytokine productionType 1 diabetes progressionPancreatic isletsType 1 diabetes mellitusMild insulin resistanceDevelopment of diabetesType 2 diabetes phenotypeΒ-cell damageDirect cytotoxic effectNF-κB inhibitorAdipocyte-specific deletionProgression of T1DMDiabetes mellitusGlucose intolerancePancreatic inflammationProinflammatory cytokinesCCL5 expressionInsulin resistanceDiabetes progressionInflammatory responseNF-κBDiabetes phenotypeMice exhibit
2014
Carbamoylating Activity Associated with the Activation of the Antitumor Agent Laromustine Inhibits Angiogenesis by Inducing ASK1-Dependent Endothelial Cell Death
Ji W, Yang M, Praggastis A, Li Y, Zhou HJ, He Y, Ghazvinian R, Cincotta DJ, Rice KP, Min W. Carbamoylating Activity Associated with the Activation of the Antitumor Agent Laromustine Inhibits Angiogenesis by Inducing ASK1-Dependent Endothelial Cell Death. PLOS ONE 2014, 9: e103224. PMID: 25068797, PMCID: PMC4113355, DOI: 10.1371/journal.pone.0103224.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntineoplastic AgentsBiocatalysisCarbamatesCattleCell DeathCells, CulturedEndothelial CellsHumansHydrazinesImmunoblottingIsocyanatesMAP Kinase Kinase Kinase 5Mitogen-Activated Protein Kinase 8Neovascularization, PhysiologicSignal TransductionSulfonamidesThioredoxin Reductase 1ThioredoxinsConceptsTrx reductase 1Non-apoptotic pathwaysEndothelial cellsKinase activityCys residuesASK1Endothelial cell deathReductase 1Cell deathInterstrand DNATrx1Reduced thioredoxinInduces dissociationCell lysatesInhibitor 14Role of methylWhole cellsInhibits angiogenesisInhibition of angiogenesisEC deathFree thiol groupsChloroethylating speciesTumor angiogenesisASK1-JNK/p38Cells