Carbamoylating Activity Associated with the Activation of the Antitumor Agent Laromustine Inhibits Angiogenesis by Inducing ASK1-Dependent Endothelial Cell Death
Ji W, Yang M, Praggastis A, Li Y, Zhou HJ, He Y, Ghazvinian R, Cincotta DJ, Rice KP, Min W. Carbamoylating Activity Associated with the Activation of the Antitumor Agent Laromustine Inhibits Angiogenesis by Inducing ASK1-Dependent Endothelial Cell Death. PLOS ONE 2014, 9: e103224. PMID: 25068797, PMCID: PMC4113355, DOI: 10.1371/journal.pone.0103224.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntineoplastic AgentsBiocatalysisCarbamatesCattleCell DeathCells, CulturedEndothelial CellsHumansHydrazinesImmunoblottingIsocyanatesMAP Kinase Kinase Kinase 5Mitogen-Activated Protein Kinase 8Neovascularization, PhysiologicSignal TransductionSulfonamidesThioredoxin Reductase 1ThioredoxinsConceptsTrx reductase 1Non-apoptotic pathwaysEndothelial cellsKinase activityCys residuesASK1Endothelial cell deathReductase 1Cell deathInterstrand DNATrx1Reduced thioredoxinInduces dissociationCell lysatesInhibitor 14Role of methylWhole cellsInhibits angiogenesisInhibition of angiogenesisEC deathFree thiol groupsChloroethylating speciesTumor angiogenesisASK1-JNK/p38Cells