Retro-orbital injection of FITC-dextran is an effective and economical method for observing mouse retinal vessels.
Li S, Li T, Luo Y, Yu H, Sun Y, Zhou H, Liang X, Huang J, Tang S. Retro-orbital injection of FITC-dextran is an effective and economical method for observing mouse retinal vessels. Molecular Vision 2011, 17: 3566-73. PMID: 22219652, PMCID: PMC3250377.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornDextransFluorescein AngiographyFluorescein-5-isothiocyanateHeart VentriclesHumansImage Processing, Computer-AssistedInfant, NewbornInjections, IntraocularMiceMice, Inbred C57BLMolecular ImagingOxygenPerfusionRetinaRetinal VesselsRetinopathy of PrematurityReverse Transcriptase Polymerase Chain ReactionSoftwareConceptsMouse retinal vesselsRO injectionOIR miceOxygen-induced retinopathyRetro-orbital injectionLV perfusionRetinal vesselsFITC-dextranTotal retina areaArea of neovascularizationRetinal flatmountsRetinal neovascularizationVentricular perfusionNormal miceFluorescein isothiocyanate-dextranRetina areaLeft ventriclePostnatal micePerfusionMiceOrbital injectionInjectionSignificant differencesNeovascularizationVessels