Blocking Fas-signaling in adipocytes and hepatocytes prevents obesity-associated inflammation, insulin resistance, and hepatosteatosis
Bae S, Ullah I, Beloor J, Lim J, Chung K, Yi Y, Kang E, Yun G, Rhim T, Lee S. Blocking Fas-signaling in adipocytes and hepatocytes prevents obesity-associated inflammation, insulin resistance, and hepatosteatosis. Journal Of Industrial And Engineering Chemistry 2024, 135: 434-443. DOI: 10.1016/j.jiec.2024.01.055.Peer-Reviewed Original ResearchFas signalingObese mouse modelInhibit Fas signalingFas-mediated apoptosisObesity-associated inflammationFas-signalingInsulin resistanceAdipose tissueMouse modelHepatic steatosisMetabolic dysfunctionSystemic free fatty acidsPro-inflammatory cytokine secretionReduced apoptosisApoptosisFatty acidsReversible inflammationSystemic deliveryCytokine secretionLiver functionInflammatory pathwaysFree fatty acidsLiver steatosisInflammationHepatic triglycerideBlocking Fas signaling in adipose tissue ameliorates obesity-associated inflammation, insulin resistance, and hepatosteatosis
Beloor J, Ullah I, Kim J, Choi C, Kim S, Chung K, KR J, Yi Y, Rhim T, Kumar P, Lee S. Blocking Fas signaling in adipose tissue ameliorates obesity-associated inflammation, insulin resistance, and hepatosteatosis. Journal Of Pharmaceutical Investigation 2024, 54: 519-537. DOI: 10.1007/s40005-024-00668-9.Peer-Reviewed Original ResearchFas signalingMatrix-assisted laser desorption/ionization-time of flight spectrometryBlock Fas signalingNon-apoptotic signalsMatrix-assisted laser desorption/ionization-timeNon-apoptotic pathwaysAdipose tissue macrophagesHigh-fat diet-induced obese mouse modelImpaired insulin signalingInsulin resistanceDiet-induced obesity mouse modelDUTP nick end labeling assayRecruitment of adipose tissue macrophagesFas receptorInsulin signalingPeptide polymerizationNick end labeling assayTissue macrophagesAdipocyte apoptosisSuppress production of inflammatory cytokinesTerminal deoxynucleotidyl transferase dUTP nick end labeling assayReal-time PCRDeoxynucleotidyl transferase dUTP nick end labeling assayObese mouse modelGene expressionIntranasal Delivery of Anti-Apoptotic siRNA Complexed with Fas-Signaling Blocking Peptides Attenuates Cellular Apoptosis in Brain Ischemia
Chung K, Ullah I, Yi Y, Kang E, Yun G, Heo S, Kim M, Chung S, Park S, Lim J, Lee M, Rhim T, Lee S. Intranasal Delivery of Anti-Apoptotic siRNA Complexed with Fas-Signaling Blocking Peptides Attenuates Cellular Apoptosis in Brain Ischemia. Pharmaceutics 2024, 16: 290. PMID: 38399343, PMCID: PMC10892455, DOI: 10.3390/pharmaceutics16020290.Peer-Reviewed Original ResearchExtrinsic apoptosis pathwayIntrinsic apoptosis pathwayApoptosis pathwayNeuronal cell deathRelease of cytochrome c.Cell deathInhibit Fas signalingNona-arginine peptideFas-signalingFas signalingConfocal microscopy analysisMitochondria disruptionMiddle cerebral artery occlusionFas receptorIntrinsic pathwayTargeting BaxCytochrome c.Molecular mechanismsCellular apoptosisLigand signalingBaxBrain ischemiaPathwayTreatment of brain ischemiaSiRNA complexes