1995
The hook protein of Borrelia burgdorferi, encoded by the flgE gene, is serologically recognized in Lyme disease.
Jwang B, Dewing P, Fikrig E, Flavell RA. The hook protein of Borrelia burgdorferi, encoded by the flgE gene, is serologically recognized in Lyme disease. MSphere 1995, 2: 609-15. PMID: 8548542, PMCID: PMC170207, DOI: 10.1128/cdli.2.5.609-615.1995.Peer-Reviewed Original ResearchConceptsFlgE proteinFlgE geneEscherichia coli expression plasmidPrimary protein sequenceB. burgdorferi N40Hook geneWhole cell lysatesHook proteinLinear chromosomesPeriplasmic flagellaProtein sequencesFlgERecombinant proteinsB. burgdorferiBasal bodiesFlagellar filamentsWestern blot analysisBorrelia burgdorferiGenesExpression plasmidProteinBlot analysisNegative bacteriaMurine antiseraLyme disease
1992
Comparison of whole-cell antibodies and an antigenic flagellar epitope of Borrelia burgdorferi in serologic tests for diagnosis of Lyme borreliosis
Magnarelli LA, Fikrig E, Berland R, Anderson JF, Flavell RA. Comparison of whole-cell antibodies and an antigenic flagellar epitope of Borrelia burgdorferi in serologic tests for diagnosis of Lyme borreliosis. Journal Of Clinical Microbiology 1992, 30: 3158-3162. PMID: 1280650, PMCID: PMC270607, DOI: 10.1128/jcm.30.12.3158-3162.1992.Peer-Reviewed Original ResearchConceptsWhole-cell B. burgdorferiEnzyme-linked immunosorbent assayLyme borreliosisB. burgdorferiRecombinant flagellar proteinsBorrelia burgdorferiSecond study groupFlagellar epitopesErythema migransDetectable antibodiesSerologic testsTreponemal antibodiesRecombinant proteinsStudy groupImmunosorbent assayAntibodiesAntigenCausative agentAntigenic regionsBorreliosisBurgdorferiComparable sensitivityHuman serumMigransSerum