2012
Alignment and Calibration of Total Internal Reflection Fluorescence Microscopy Systems
Toomre D. Alignment and Calibration of Total Internal Reflection Fluorescence Microscopy Systems. Cold Spring Harbor Protocols 2012, 2012: pdb.prot068668. PMID: 22474669, DOI: 10.1101/pdb.prot068668.Peer-Reviewed Original ResearchConceptsTotal internal reflection fluorescence microscopyThinner optical planeDynamic cellular processesCell fluorescent microscopyReflection fluorescence microscopyMembrane traffickingCellular processesCytoskeleton remodelingCell cortexCell signalingOrganelle levelTransient intermediate statesMolecular manipulationFluorescence microscopyRefractive indexFluorescent microscopyHigh numerical aperture objective lensFluorescence microscopy systemNumerical aperture objective lensConventional epifluorescence microscopeEpifluorescence microscopeDifferent refractive indicesCellular samplesRecent availabilityConfocal microscope
2011
3-D Reconstruction of Microtubules from Multi-Angle Total Internal Reflection Fluorescence Microscopy Using Bayesian Framework
Yang Q, Karpikov A, Toomre D, Duncan JS. 3-D Reconstruction of Microtubules from Multi-Angle Total Internal Reflection Fluorescence Microscopy Using Bayesian Framework. IEEE Transactions On Image Processing 2011, 20: 2248-2259. PMID: 21324778, DOI: 10.1109/tip.2011.2114359.Peer-Reviewed Original ResearchConceptsEvanescent fieldDifferent penetration depthsTIRF imagesPenetration depthSamples of microtubulesLaser beamTotal internal reflection fluorescence microscopyAxial resolutionReflection fluorescence microscopyLarge radiusIncident angleMulti-angle total internal reflection fluorescence microscopyElectron microscopy imagesTIRF dataSmall radiusTracking of microtubulesMicroscopy imagesFluorescence microscopyMicroscopyRadiusReconstruction resultsCurvilinear characteristicsZ-dimensionExperimental calibrationMicrotubule curvature