Alignment and Calibration of Total Internal Reflection Fluorescence Microscopy Systems
Toomre D. Alignment and Calibration of Total Internal Reflection Fluorescence Microscopy Systems. Cold Spring Harbor Protocols 2012, 2012: pdb.prot068668. PMID: 22474669, DOI: 10.1101/pdb.prot068668.Peer-Reviewed Original ResearchConceptsTotal internal reflection fluorescence microscopyThinner optical planeDynamic cellular processesCell fluorescent microscopyReflection fluorescence microscopyMembrane traffickingCellular processesCytoskeleton remodelingCell cortexCell signalingOrganelle levelTransient intermediate statesMolecular manipulationFluorescence microscopyRefractive indexFluorescent microscopyHigh numerical aperture objective lensFluorescence microscopy systemNumerical aperture objective lensConventional epifluorescence microscopeEpifluorescence microscopeDifferent refractive indicesCellular samplesRecent availabilityConfocal microscope