2023
Targeted and selective knockout of the TLQP-21 neuropeptide unmasks its unique role in energy homeostasis
Sahu B, Razzoli M, McGonigle S, Pallais J, Nguyen M, Sadahiro M, Jiang C, Lin W, Kelley K, Rodriguez P, Mansk R, Cero C, Caviola G, Palanza P, Rao L, Beetch M, Alejandro E, Sham Y, Frontini A, Salton S, Bartolomucci A. Targeted and selective knockout of the TLQP-21 neuropeptide unmasks its unique role in energy homeostasis. Molecular Metabolism 2023, 76: 101781. PMID: 37482186, PMCID: PMC10400922, DOI: 10.1016/j.molmet.2023.101781.Peer-Reviewed Original ResearchConceptsGenetic engineering approachesUnique metabolic phenotypeMass spectrometry identificationPrecursor geneGel digestionGenetic lossTLQP-21Cleavage siteC-terminal arginineGenesMutant sequencesSelective knockoutEssential roleBiological constraintsMetabolic phenotypeMouse linesEnergy homeostasisComposite phenotypeMutant miceValuable resourceVGF gene
2015
Role of VGF-Derived Carboxy-Terminal Peptides in Energy Balance and Reproduction: Analysis of “Humanized” Knockin Mice Expressing Full-Length or Truncated VGF
Sadahiro M, Erickson C, Lin WJ, Shin AC, Razzoli M, Jiang C, Fargali S, Gurney A, Kelley KA, Buettner C, Bartolomucci A, Salton SR. Role of VGF-Derived Carboxy-Terminal Peptides in Energy Balance and Reproduction: Analysis of “Humanized” Knockin Mice Expressing Full-Length or Truncated VGF. Endocrinology 2015, 156: 1724-1738. PMID: 25675362, PMCID: PMC4398760, DOI: 10.1210/en.2014-1826.Peer-Reviewed Original ResearchConceptsEnergy expenditureRole of VGFWild-type miceKnockin mouse modelGlucose toleranceFemale miceGlucose homeostasisMouse modelBody weightReproductive functionKnockin miceVGF peptidesMiceVGF proteinMetabolic phenotypeInfertile miceSingle nucleotide polymorphismsFat storageTerminal peptidesTargeted deletionCarboxy-terminal peptideVGFObesityCritical regulatorC-terminal region