2008
Thrombospondin-1-Induced Migration Is Functionally Dependent Upon Focal Adhesion Kinase
Wang XJ, Maier K, Fuse S, Willis AI, Olson E, Nesselroth S, Sumpio BE, Gahtan V. Thrombospondin-1-Induced Migration Is Functionally Dependent Upon Focal Adhesion Kinase. Vascular And Endovascular Surgery 2008, 42: 256-262. PMID: 18319354, DOI: 10.1177/1538574408314440.Peer-Reviewed Original Research
2007
Phosphatase PTEN is inactivated in bovine aortic endothelial cells exposed to cyclic strain
Hoshino Y, Nishimura K, Sumpio BE. Phosphatase PTEN is inactivated in bovine aortic endothelial cells exposed to cyclic strain. Journal Of Cellular Biochemistry 2007, 100: 515-526. PMID: 16927376, DOI: 10.1002/jcb.21085.Peer-Reviewed Original ResearchConceptsCasein kinase 2Transfection of ECsPTEN plasmidVascular cell morphologyEndothelial cellsPI3K activitySuppression of apoptosisPI3K-Akt pathwayLipid phosphataseMaximal activityPhosphatase PTENPhospho-PTENPTEN activityBovine aortic endothelial cellsIntracellular phosphatidylinositolUpstream regulatorAkt activityKinase 2Phospho-AKT activityK activityTime-dependent mannerAortic endothelial cellsCell morphologyCell proliferationPTEN
2006
Role of AKT in cyclic strain-induced endothelial cell proliferation and survival
Nishimura K, Li W, Hoshino Y, Kadohama T, Asada H, Ohgi S, Sumpio BE. Role of AKT in cyclic strain-induced endothelial cell proliferation and survival. American Journal Of Physiology - Cell Physiology 2006, 290: c812-c821. PMID: 16469863, DOI: 10.1152/ajpcell.00347.2005.Peer-Reviewed Original ResearchConceptsEndothelial cellsCycles/minRole of AktEC proliferationCultured bovine aortic endothelial cellsSurvival of ECsAortic endothelial cellsApoptotic endothelial cellsGSK-3betaBovine aortic endothelial cellsEndothelial cell proliferationFrequency of strainsPositive cellsGlycogen synthase kinaseKinase-dead AktCell proliferationCaspase-3AktSurvivalCell numberSynthase kinaseProliferationEarly phosphorylationBad phosphorylation
2005
Differential responsiveness of early- and late-passage endothelial cells to shear stress
Kudo FA, Warycha B, Juran PJ, Asada H, Teso D, Aziz F, Frattini J, Sumpio BE, Nishibe T, Cha C, Dardik A. Differential responsiveness of early- and late-passage endothelial cells to shear stress. The American Journal Of Surgery 2005, 190: 763-769. PMID: 16226955, DOI: 10.1016/j.amjsurg.2005.07.017.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAortaApoptosisBlotting, WesternCattleCell CountCell DivisionCell ProliferationCells, CulturedEndothelium, VascularIn Vitro TechniquesMuscle, Smooth, VascularPhosphorylationProliferating Cell Nuclear AntigenProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktShear StrengthStress, MechanicalTumor Suppressor Protein p53ConceptsLate passage endothelial cellsOrbital shear stressEarly passage cellsSmooth muscle cell migrationMuscle cell migrationEndothelial cellsSenescence modelAkt phosphorylationCell migrationProtein kinase B activationPassage cellsKinase B activationCell proliferationVascular disease increasesLate passage cellsBovine aortic endothelial cellsNuclear antigen reactivityAortic endothelial cellsEndothelial cell proliferationNeointimal hyperplasiaAntigen reactivityTotal AktBoyden chamberB activationWestern blottingThe role of STAT-3 in the mediation of smooth muscle cell response to cyclic strain
Kakisis JD, Pradhan S, Cordova A, Liapis CD, Sumpio BE. The role of STAT-3 in the mediation of smooth muscle cell response to cyclic strain. The International Journal Of Biochemistry & Cell Biology 2005, 37: 1396-1406. PMID: 15833272, DOI: 10.1016/j.biocel.2005.01.009.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCell ProliferationCells, CulturedDNA-Binding ProteinsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Myocytes, Smooth MusclePhosphorylationPyrazolesPyrimidinesRatsSerineSignal TransductionSrc-Family KinasesSTAT3 Transcription FactorStress, MechanicalTrans-ActivatorsTyrosineConceptsSerine phosphorylationTyrosine phosphorylationSmooth muscle cellsSTAT-3Vascular smooth muscle cellsSTAT-3 tyrosine phosphorylationSpecific inhibitorVascular cell morphologyExtracellular signal-regulated kinase 1/2Extracellular mechanical signalsSignal-regulated kinase 1/2Basal serine phosphorylationInhibition of SrcA7r5 smooth muscle cellsNegative feedback loopInhibition of ERK1/2Cells sensePhosphospecific antibodiesPhosphatidylinositol 3Signal transducerTranscription 3Kinase 2Kinase 1/2Smooth muscle cell responseBasal phosphorylationDifferential effects of orbital and laminar shear stress on endothelial cells
Dardik A, Chen L, Frattini J, Asada H, Aziz F, Kudo FA, Sumpio BE. Differential effects of orbital and laminar shear stress on endothelial cells. Journal Of Vascular Surgery 2005, 41: 869-880. PMID: 15886673, DOI: 10.1016/j.jvs.2005.01.020.Peer-Reviewed Original Research
2004
Strain-induced vascular endothelial cell proliferation requires PI3K-dependent mTOR-4E-BP1 signal pathway
Li W, Sumpio BE. Strain-induced vascular endothelial cell proliferation requires PI3K-dependent mTOR-4E-BP1 signal pathway. AJP Heart And Circulatory Physiology 2004, 288: h1591-h1597. PMID: 15591103, DOI: 10.1152/ajpheart.00382.2004.Peer-Reviewed Original ResearchMeSH Keywords3-Phosphoinositide-Dependent Protein KinasesAndrostadienesAnimalsAntibiotics, AntineoplasticAortaCarrier ProteinsCattleCell DivisionCells, CulturedChromonesEndothelium, VascularEnzyme InhibitorsFlavonoidsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3MorpholinesPhosphatidylinositol 3-KinasesPhosphodiesterase InhibitorsPhosphoinositide-3 Kinase InhibitorsPhosphoproteinsPhosphorylationProtein KinasesProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktRibosomal Protein S6 KinasesSignal TransductionSirolimusTOR Serine-Threonine KinasesWortmanninConceptsVascular endothelial cell proliferationEndothelial cell proliferationStrain-induced activationSignal pathwayEC proliferationPD 98059Cell proliferationPI3K inhibitor wortmanninPI3K inhibitorsCycles/minExtracellular signal-regulated kinases 1Inhibitor PD 98059MTOR pathwaySignal-regulated kinases 1Bovine aortic ECsMammalian targetMTOR-4EK inhibitorsEukaryotic initiation factor 4EAortic ECsInitiation factor 4EMEK1 inhibitor PD 98059K activationProliferationRapamycin
2003
Involvement of S6 kinase and p38 mitogen activated protein kinase pathways in strain‐induced alignment and proliferation of bovine aortic smooth muscle cells
Li W, Chen Q, Mills I, Sumpio BE. Involvement of S6 kinase and p38 mitogen activated protein kinase pathways in strain‐induced alignment and proliferation of bovine aortic smooth muscle cells. Journal Of Cellular Physiology 2003, 195: 202-209. PMID: 12652647, DOI: 10.1002/jcp.10230.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell DivisionCells, CulturedDNA-Binding ProteinsEnzyme InhibitorsImidazolesImmunosuppressive AgentsMAP Kinase Signaling SystemMitogen-Activated Protein KinasesMuscle, Smooth, VascularMyocytes, Smooth MuscleP38 Mitogen-Activated Protein KinasesPyridinesRegional Blood FlowRibosomal Protein S6 KinasesSirolimusStress, MechanicalTranscription FactorsConceptsAortic smooth muscle cellsBovine aortic smooth muscle cellsKinase pathwaySmooth muscle cell phenotypeSmooth muscle cellsStrain-induced proliferationBovine aortic SMCS6 kinaseMuscle cell phenotypeSpecific inhibitorProliferation of SMCsActivation of p38SMC proliferationAortic SMCsMuscle cellsSMC alignmentModulation of vascular smooth muscle cell alignment by cyclic strain is dependent on reactive oxygen species and P38 mitogen-activated protein kinase
Chen Q, Li W, Quan Z, Sumpio BE. Modulation of vascular smooth muscle cell alignment by cyclic strain is dependent on reactive oxygen species and P38 mitogen-activated protein kinase. Journal Of Vascular Surgery 2003, 37: 660-668. PMID: 12618707, DOI: 10.1067/mva.2003.95.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCells, CulturedEnzyme ActivationEnzyme InhibitorsImidazolesJNK Mitogen-Activated Protein KinasesMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesMultienzyme ComplexesMuscle, Smooth, VascularNADH, NADPH OxidoreductasesNADPH OxidasesOnium CompoundsOxidation-ReductionP38 Mitogen-Activated Protein KinasesPhosphorylationPyridinesReactive Oxygen SpeciesStress, MechanicalRole of PP2A in the regulation of p38 MAPK activation in bovine aortic endothelial cells exposed to cyclic strain
Lee T, Kim SJ, Sumpio BE. Role of PP2A in the regulation of p38 MAPK activation in bovine aortic endothelial cells exposed to cyclic strain. Journal Of Cellular Physiology 2003, 194: 349-355. PMID: 12548554, DOI: 10.1002/jcp.10211.Peer-Reviewed Original ResearchConceptsP38 MAPK activationMAPK activationCatalytic subunitBovine aortic endothelial cellsPhospho-p38 MAPKOkadaic acidProtein phosphatase type 2AInhibitor of PP2AAortic endothelial cellsEndothelial cellsProtein kinaseRapid phosphorylationPP2AP38 mitogenRegulatory roleCell lysatesPhosphatase activityPP2AcMAPKPhosphorylationCyclic strainSubunitsType 2ACyclic strain resultsCells
2000
Endothelial cell response to different mechanical forces
Azuma N, Duzgun S, Ikeda M, Kito H, Akasaka N, Sasajima T, Sumpio B. Endothelial cell response to different mechanical forces. Journal Of Vascular Surgery 2000, 32: 789-794. PMID: 11013043, DOI: 10.1067/mva.2000.107989.Peer-Reviewed Original ResearchAnimalsAortaCattleCells, CulturedEndothelium, VascularEnzyme ActivationFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesFocal AdhesionsHumansImmunoblottingJNK Mitogen-Activated Protein KinasesMechanoreceptorsMitogen-Activated Protein KinasesPhosphorylationProtein-Tyrosine KinasesSignal TransductionStress, MechanicalHomocysteine stimulates MAP kinase in bovine aortic smooth muscle cells
Woo D, Dudrick S, Sumpio B. Homocysteine stimulates MAP kinase in bovine aortic smooth muscle cells. Surgery 2000, 128: 59-66. PMID: 10876187, DOI: 10.1067/msy.2000.106531.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell DivisionCells, CulturedDose-Response Relationship, DrugEnzyme ActivationEnzyme InhibitorsFlavonoidsHomocysteineMAP Kinase Signaling SystemMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesMuscle, Smooth, VascularPhosphorylationConceptsMAP kinase phosphorylationMAP kinaseKinase phosphorylationBASMC proliferationBovine aortic smooth muscle cellsAortic smooth muscle cellsCell proliferationExtracellular signal-regulated protein kinases 1Mitogen-activated protein kinaseSmooth muscle cell proliferationProtein kinase 1Smooth muscle cellsMuscle cell proliferationMAP kinase activationDegree of phosphorylationL-homocysteineMuscle cellsProtein kinaseKinase studiesKinase activationKinase 1Hallmark of atherosclerosisKinasePhosphorylationActive formRole of Caveolin in Hemodynamic Force-Mediated Endothelial Changes
Fujioka K, Azuma N, Kito H, Gahtan V, Esato K, Sumpio B. Role of Caveolin in Hemodynamic Force-Mediated Endothelial Changes. Journal Of Surgical Research 2000, 92: 7-10. PMID: 10864474, DOI: 10.1006/jsre.2000.5838.Peer-Reviewed Original ResearchEffects of increased ambient pressure on colon cancer cell adhesion
Basson M, Yu C, Herden‐Kirchoff O, Ellermeier M, Sanders M, Merrell R, Sumpio B. Effects of increased ambient pressure on colon cancer cell adhesion. Journal Of Cellular Biochemistry 2000, 78: 47-61. PMID: 10797565, DOI: 10.1002/(sici)1097-4644(20000701)78:1<47::aid-jcb5>3.0.co;2-m.Peer-Reviewed Original ResearchConceptsColon cancer cell adhesionCancer cell adhesionTyrosine phosphorylationCell adhesionPressure-stimulated adhesionFocal adhesion kinaseHuman colon cancer cellsIntestinal epithelial biologyColon cancer cellsColon cancer cell linesHuman colon cancer cell linesBeta1 integrin subunitsHuman colon cancerAdhesion kinaseBovine aortic smooth muscle cellsAortic smooth muscle cellsIntracellular proteinsEpithelial biologyMatrix proteinsAbsence of serumIntegrin subunitsCancer cell linesPrimary cellsSmooth muscle cellsKinase inhibitionTranslocation of PKC isoforms in bovine aortic smooth muscle cells exposed to strain
Han O, Takei T, Basson M, Sumpio B. Translocation of PKC isoforms in bovine aortic smooth muscle cells exposed to strain. Journal Of Cellular Biochemistry 2000, 80: 367-372. PMID: 11135367, DOI: 10.1002/1097-4644(20010301)80:3<367::aid-jcb100>3.0.co;2-2.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsAortic smooth muscle cellsBovine aortic smooth muscle cellsMuscle cellsPKC-alphaPKC isoformsCyclic strain resultsCycles/minCytosolic fractionProliferation responseParticulate fractionNF-kappaBProtein kinase C activityCollagen type ICellular proliferationSignificant stimulationImmunoblotting analysisKinase C activityType IPKC activityC activityStimulationIndividual PKC isoformsIsoforms
1999
Phosphatidylinositol-3 Kinase Dependent MAP Kinase Activation via p21ras in Endothelial Cells Exposed to Cyclic Strain
Ikeda M, Kito H, Sumpio B. Phosphatidylinositol-3 Kinase Dependent MAP Kinase Activation via p21ras in Endothelial Cells Exposed to Cyclic Strain. Biochemical And Biophysical Research Communications 1999, 257: 668-671. PMID: 10208841, DOI: 10.1006/bbrc.1999.0532.Peer-Reviewed Original ResearchMeSH KeywordsAndrostadienesAnimalsAortaCalcium-Calmodulin-Dependent Protein KinasesCattleCells, CulturedChromonesEndothelium, VascularEnzyme ActivationGuanosine TriphosphateHemodynamicsMorpholinesOncogene Protein p21(ras)Phosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPhosphorylationSignal TransductionStress, MechanicalTime FactorsWortmanninConceptsERK1/2 activationPhosphatidylinositol-3 kinase inhibitorMAP kinase activationExtracellular signal-regulated protein kinases 1Endothelial cellsMechanical stretchKinase inhibitorsERK1/2 phosphorylationEffect of wortmanninPossible involvementHemodynamic forcesProtein kinase 1ActivationKinase 1Upstream pathwaysRapid activationKinase activationSignal transduction pathwaysP21ras activationLY294002ERK1 activationERK2 activation
1998
Cyclooxygenase Expression in Bovine Aortic Endothelial Cells Exposed to Cyclic Strain
Kito H, Yokoyama C, Inoue H, Tanabe T, Nakajima N, Sumpio B. Cyclooxygenase Expression in Bovine Aortic Endothelial Cells Exposed to Cyclic Strain. Endothelium 1998, 6: 107-112. PMID: 9930644, DOI: 10.3109/10623329809072197.Peer-Reviewed Original ResearchConceptsAortic endothelial cellsBovine aortic endothelial cellsEndothelial cellsInduction of COXCOX-1 expressionCOX-2 promoter activityExposure of ECsPromoter activityCycles/minCOX-2Cyclooxygenase expressionCOX-1Weak inducerMRNA expressionExtent of inductionNorthern blot analysisBlot analysisTransient transfection experimentsLipopolysaccharideMinimal effectExpressionInductionTransfection experimentsCyclic strainCells
1997
Cyclic Strain Is a Weak Inducer of Prostacyclin Synthase Expression in Bovine Aortic Endothelial Cells
Segurola R, Oluwole B, Mills I, Yokoyama C, Tanabe T, Kito H, Nakajima N, Sumpio B. Cyclic Strain Is a Weak Inducer of Prostacyclin Synthase Expression in Bovine Aortic Endothelial Cells. Journal Of Surgical Research 1997, 69: 135-138. PMID: 9202659, DOI: 10.1006/jsre.1997.5050.Peer-Reviewed Original ResearchStrain activation of bovine aortic smooth muscle cell proliferation and alignment: Study of strain dependency and the role of protein kinase A and C signaling pathways
Mills I, Cohen C, Kamal K, Li G, Shin T, Du W, Sumpio B. Strain activation of bovine aortic smooth muscle cell proliferation and alignment: Study of strain dependency and the role of protein kinase A and C signaling pathways. Journal Of Cellular Physiology 1997, 170: 228-234. PMID: 9066778, DOI: 10.1002/(sici)1097-4652(199703)170:3<228::aid-jcp2>3.0.co;2-q.Peer-Reviewed Original ResearchMeSH KeywordsActivating Transcription Factor 2Adenylyl CyclasesAnimalsAortaCattleCell DivisionCells, CulturedCyclic AMPCyclic AMP Response Element-Binding ProteinCyclic AMP-Dependent Protein KinasesEndothelium, VascularLeucine ZippersMuscle, Smooth, VascularProtein Kinase CSignal TransductionStress, MechanicalTranscription FactorsConceptsBovine aortic SMCSMC proliferationCAMP response elementAortic SMCsAC/cAMP/PKAAortic smooth muscle cell proliferationSmooth muscle cell proliferationProtein levelsCyclic AMP accumulationMuscle cell proliferationSmooth muscle cell phenotypeExposure of SMCAdenylate cyclase activityMuscle cell phenotypeAMP accumulationInhibition of PKACAMP/PKACyclic AMP pathwayMultifactorial natureProtein kinase C activityCyclase activityProtein kinase A (PKA) activityLack of involvementEnhanced proliferationCell proliferation
1996
Tyrosine phosphorylation of pp125FAK and paxillin in aortic endothelial cells induced by mechanical strain
Yano Y, Geibel J, Sumpio BE. Tyrosine phosphorylation of pp125FAK and paxillin in aortic endothelial cells induced by mechanical strain. American Journal Of Physiology 1996, 271: c635-c649. PMID: 8770005, DOI: 10.1152/ajpcell.1996.271.2.c635.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell Adhesion MoleculesCell MovementCells, CulturedCytoskeletal ProteinsEndothelium, VascularFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGenisteinIsoflavonesNitrilesPaxillinPhosphoproteinsPhosphorylationPhosphotyrosineProtein-Tyrosine KinasesStress, MechanicalTissue DistributionTyrosineTyrphostins