2018
Regulation of Yes-Associated Protein by Laminar Flow
Chitragari G, Shalaby SY, Sumpio BJ, Kurita J, Sumpio BE. Regulation of Yes-Associated Protein by Laminar Flow. Annals Of Vascular Surgery 2018, 52: 183-191. PMID: 29758328, DOI: 10.1016/j.avsg.2018.03.002.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingCell ShapeCells, CulturedCytoskeletonDown-RegulationHuman Umbilical Vein Endothelial CellsHumansMechanotransduction, CellularPhosphoproteinsPhosphorylationProteolysisPulsatile FlowRegional Blood FlowStress, MechanicalTime FactorsTranscription FactorsYAP-Signaling ProteinsConceptsHuman umbilical vein endothelial cellsTotal YAPExposure of HUVECsRelease of mediatorsYAP levelsSignificant decreaseUbiquitin-proteasome system inhibitorsUmbilical vein endothelial cellsEndothelial cell proliferationVein endothelial cellsVascular healthSystem inhibitorsYes-Associated ProteinDisease modulationIntimal hyperplasiaDisturbed flowEndothelial cellsBlood vesselsDisease statesPotential targetCell proliferationProteasome-independent mechanismExposureKey regulatorPYAP
2013
PECAM-1 phosphorylation and tissue factor expression in HUVECs exposed to uniform and disturbed pulsatile flow and chemical stimuli
Moriguchi T, Sumpio BE. PECAM-1 phosphorylation and tissue factor expression in HUVECs exposed to uniform and disturbed pulsatile flow and chemical stimuli. Journal Of Vascular Surgery 2013, 61: 481-488. PMID: 24342062, DOI: 10.1016/j.jvs.2013.09.059.Peer-Reviewed Original Research
2011
The role of mechanical forces and adenosine in the regulation of intestinal enterochromaffin cell serotonin secretion
Chin A, Svejda B, Gustafsson B, Granlund A, Sandvik A, Timberlake A, Sumpio B, Pfragner R, Modlin I, Kidd M. The role of mechanical forces and adenosine in the regulation of intestinal enterochromaffin cell serotonin secretion. AJP Gastrointestinal And Liver Physiology 2011, 302: g397-g405. PMID: 22038827, PMCID: PMC3287403, DOI: 10.1152/ajpgi.00087.2011.Peer-Reviewed Original ResearchMeSH KeywordsAcetamidesAdenosineAdenosine A2 Receptor AgonistsAdenosine A2 Receptor AntagonistsAdenosine-5'-(N-ethylcarboxamide)AdultAgedCell Line, TumorCells, CulturedColonCrohn DiseaseCyclic AMPCyclic AMP Response Element-Binding ProteinCyclic AMP-Dependent Protein KinasesEnterochromaffin CellsFemaleGene ExpressionHumansMaleMAP Kinase Kinase 1MAP Kinase Signaling SystemMechanotransduction, CellularMiddle AgedPhosphorylationProto-Oncogene Proteins c-aktPurinesReceptor, Adenosine A1Receptor, Adenosine A2AReceptor, Adenosine A2BReceptor, Adenosine A3SerotoninSignal TransductionStress, MechanicalTryptophan HydroxylaseVesicular Monoamine Transport ProteinsConceptsInflammatory bowel diseaseEC cellsCell functionEC cell functionMechanical stimulationDevelopment of agentsSecrete serotoninBowel diseaseIntracellular cAMP levelsGut motilityEnterochromaffin cellsAdenosine responsivenessReceptor agonistReceptor expressionHuman EC cellsSerotonin secretionAdenosine receptorsMRS1754CAMP productionSecretionCAMP levelsNECANeoplasiaMechanosensory cellsDiseaseVarying Effects of Hemodynamic Forces on Tissue Factor RNA Expression in Human Endothelial Cells
Abe R, Yamashita N, Rochier A, Nixon A, Abe R, Madri JA, Sumpio BE. Varying Effects of Hemodynamic Forces on Tissue Factor RNA Expression in Human Endothelial Cells. Journal Of Surgical Research 2011, 170: 150-156. PMID: 21592524, DOI: 10.1016/j.jss.2011.04.002.Peer-Reviewed Original ResearchMeSH KeywordsAtherosclerosisCells, CulturedEndothelial CellsHemodynamicsHumansPulsatile FlowRNA, MessengerStress, MechanicalThrombinThromboplastinConceptsLaminar flowOscillatory flowCyclic strainUnidirectional laminar flowUniform laminar flowMechanical stressDisturbed flowTF RNA expressionHuman umbilical vein endothelial cellsFlowStatic controlHigh TF expressionTF expressionForceMechanical forcesEndothelial cellsRNA expressionSustained amplificationLaminar shear, but not orbital shear, has a synergistic effect with thrombin stimulation on tissue factor expression in human umbilical vein endothelial cells
Rochier A, Nixon A, Yamashita N, Abe R, Abe R, Madri JA, Sumpio BE. Laminar shear, but not orbital shear, has a synergistic effect with thrombin stimulation on tissue factor expression in human umbilical vein endothelial cells. Journal Of Vascular Surgery 2011, 54: 480-488. PMID: 21367569, DOI: 10.1016/j.jvs.2011.01.002.Peer-Reviewed Original ResearchAnalysis of VarianceBlotting, WesternCell Culture TechniquesCells, CulturedEndothelial CellsEnzyme ActivationHumansMechanotransduction, CellularMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3P38 Mitogen-Activated Protein KinasesPhosphorylationProtein Kinase InhibitorsRNA, MessengerStress, MechanicalThrombinThromboplastinTime FactorsUp-RegulationPulsatile to-fro flow induces greater and sustained expression of tissue factor RNA in HUVEC than unidirectional laminar flow
Abe R, Yamashita N, Rochier A, Abe R, Nixon A, Madri JA, Sumpio BE. Pulsatile to-fro flow induces greater and sustained expression of tissue factor RNA in HUVEC than unidirectional laminar flow. AJP Heart And Circulatory Physiology 2011, 300: h1345-h1351. PMID: 21257914, PMCID: PMC3075035, DOI: 10.1152/ajpheart.01197.2010.Peer-Reviewed Original Research
2009
Role of integrins and focal adhesion kinase in the orientation of dermal fibroblasts exposed to cyclic strain*
Wen H, Blume PA, Sumpio BE. Role of integrins and focal adhesion kinase in the orientation of dermal fibroblasts exposed to cyclic strain*. International Wound Journal 2009, 6: 149-158. PMID: 19432665, PMCID: PMC7951581, DOI: 10.1111/j.1742-481x.2009.00591.x.Peer-Reviewed Original Research
2008
The Effect of Different Frequencies of Stretch on Human Dermal Keratinocyte Proliferation and Survival
Nishimura K, Blume P, Ohgi S, Sumpio BE. The Effect of Different Frequencies of Stretch on Human Dermal Keratinocyte Proliferation and Survival. Journal Of Surgical Research 2008, 155: 125-131. PMID: 19059608, DOI: 10.1016/j.jss.2008.07.029.Peer-Reviewed Original ResearchConceptsERK 1/2P38 MAPKProtein kinaseKeratinocyte proliferation rateCell deathAkt inhibitorCyclic stretchDNA synthesisAktTransferase-mediated dUTP nick-end labeling stainingRepetitive stretchApoptosisMAPKDUTP nick end labeling stainingProliferation rateHuman dermalCell numberNick end labeling stainingTUNEL-positive cellsDermal keratinocytesStretchTerminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) stainingBrdU stainingKeratinocyte proliferationProliferationDifferential Effects of Shear Stress and Cyclic Strain on Sp1 Phosphorylation by Protein Kinase Cζ Modulates Membrane Type 1–Matrix Metalloproteinase in Endothelial Cells
Kim JI, Cordova AC, Hirayama Y, Madri JA, Sumpio BE. Differential Effects of Shear Stress and Cyclic Strain on Sp1 Phosphorylation by Protein Kinase Cζ Modulates Membrane Type 1–Matrix Metalloproteinase in Endothelial Cells. Endothelium 2008, 15: 33-42. PMID: 18568943, PMCID: PMC2644408, DOI: 10.1080/10623320802092260.Peer-Reviewed Original ResearchConceptsSp1 phosphorylationMT1-MMP expressionPromoter sitesPKCzeta inhibitorProtein kinase CzetaAffinity of Sp1Egr-1 bindingProtein kinase CζExtracellular matrix remodelingEndothelial cell migrationSp1Cell migrationPhosphorylationMatrix remodelingProtein expressionCyclic strainExpressionMembrane typeEndothelial cellsKey roleCzetaInhibitorsCζMetalloproteinaseAffinity
2007
Phosphatase PTEN is inactivated in bovine aortic endothelial cells exposed to cyclic strain
Hoshino Y, Nishimura K, Sumpio BE. Phosphatase PTEN is inactivated in bovine aortic endothelial cells exposed to cyclic strain. Journal Of Cellular Biochemistry 2007, 100: 515-526. PMID: 16927376, DOI: 10.1002/jcb.21085.Peer-Reviewed Original ResearchConceptsCasein kinase 2Transfection of ECsPTEN plasmidVascular cell morphologyEndothelial cellsPI3K activitySuppression of apoptosisPI3K-Akt pathwayLipid phosphataseMaximal activityPhosphatase PTENPhospho-PTENPTEN activityBovine aortic endothelial cellsIntracellular phosphatidylinositolUpstream regulatorAkt activityKinase 2Phospho-AKT activityK activityTime-dependent mannerAortic endothelial cellsCell morphologyCell proliferationPTENRole of Ligand-Specific Integrins in Endothelial Cell Alignment and Elongation Induced by Cyclic Strain
Hirayama Y, Sumpio BE. Role of Ligand-Specific Integrins in Endothelial Cell Alignment and Elongation Induced by Cyclic Strain. Endothelium 2007, 14: 275-283. PMID: 18080865, DOI: 10.1080/10623320701746248.Peer-Reviewed Original ResearchConceptsEndothelial cellsP38 MAPKAnti-alpha2 integrin antibodyMorphological changesP38 mitogen-activated protein kinase (MAPK) pathwayCycles/minIntegrin-blocking antibodiesP38 MAPK pathwayMitogen-activated protein kinase pathwayAlpha 5Integrin antibodyProtein kinase pathwayMAPK pathwayAntibodiesKinase pathwayMAPKMultiple biological processesActivationIntegrinsCritical roleCyclic strainPathway
2006
Role of AKT in cyclic strain-induced endothelial cell proliferation and survival
Nishimura K, Li W, Hoshino Y, Kadohama T, Asada H, Ohgi S, Sumpio BE. Role of AKT in cyclic strain-induced endothelial cell proliferation and survival. American Journal Of Physiology - Cell Physiology 2006, 290: c812-c821. PMID: 16469863, DOI: 10.1152/ajpcell.00347.2005.Peer-Reviewed Original ResearchConceptsEndothelial cellsCycles/minRole of AktEC proliferationCultured bovine aortic endothelial cellsSurvival of ECsAortic endothelial cellsApoptotic endothelial cellsGSK-3betaBovine aortic endothelial cellsEndothelial cell proliferationFrequency of strainsPositive cellsGlycogen synthase kinaseKinase-dead AktCell proliferationCaspase-3AktSurvivalCell numberSynthase kinaseProliferationEarly phosphorylationBad phosphorylationp38 Mitogen-Activated Protein Kinase Activation in Endothelial Cell Is Implicated in Cell Alignment and Elongation Induced by Fluid Shear Stress
Kadohama T, Akasaka N, Nishimura K, Hoshino Y, Sasajima T, Sumpio BE. p38 Mitogen-Activated Protein Kinase Activation in Endothelial Cell Is Implicated in Cell Alignment and Elongation Induced by Fluid Shear Stress. Endothelium 2006, 13: 43-50. PMID: 16885066, DOI: 10.1080/10623320600660219.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesAtherosclerosisBlood PressureBlood VesselsCattleCell PolarityCell ShapeCells, CulturedDose-Response Relationship, DrugEndothelial CellsEnzyme ActivationEnzyme InhibitorsHemodynamicsMAP Kinase Signaling SystemP38 Mitogen-Activated Protein KinasesPhosphorylationRegional Blood FlowStress, MechanicalTime FactorsConceptsStatic endothelial cellsFluid shear stressP38 MAPKP38 Mitogen-Activated Protein Kinase ActivationMitogen-Activated Protein Kinase ActivationEndothelial cellsProtein kinase activationP38 MAPK inhibitor SB-203580Inhibitor SB 203580Cell alignmentProtein kinaseBovine aortic endothelial cellsLaminar shear stressKinase activationSB 203580P38 mitogenCultured bovine aortic endothelial cellsEC elongationAortic endothelial cellsMAPKCellsElongationActivationMorphometric analysisKinase
2005
Differential responsiveness of early- and late-passage endothelial cells to shear stress
Kudo FA, Warycha B, Juran PJ, Asada H, Teso D, Aziz F, Frattini J, Sumpio BE, Nishibe T, Cha C, Dardik A. Differential responsiveness of early- and late-passage endothelial cells to shear stress. The American Journal Of Surgery 2005, 190: 763-769. PMID: 16226955, DOI: 10.1016/j.amjsurg.2005.07.017.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAortaApoptosisBlotting, WesternCattleCell CountCell DivisionCell ProliferationCells, CulturedEndothelium, VascularIn Vitro TechniquesMuscle, Smooth, VascularPhosphorylationProliferating Cell Nuclear AntigenProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktShear StrengthStress, MechanicalTumor Suppressor Protein p53ConceptsLate passage endothelial cellsOrbital shear stressEarly passage cellsSmooth muscle cell migrationMuscle cell migrationEndothelial cellsSenescence modelAkt phosphorylationCell migrationProtein kinase B activationPassage cellsKinase B activationCell proliferationVascular disease increasesLate passage cellsBovine aortic endothelial cellsNuclear antigen reactivityAortic endothelial cellsEndothelial cell proliferationNeointimal hyperplasiaAntigen reactivityTotal AktBoyden chamberB activationWestern blottingSustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway
Asada H, Paszkowiak J, Teso D, Alvi K, Thorisson A, Frattini JC, Kudo FA, Sumpio BE, Dardik A. Sustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway. Journal Of Vascular Surgery 2005, 42: 772-780. PMID: 16242567, DOI: 10.1016/j.jvs.2005.05.046.Peer-Reviewed Original ResearchConceptsOrbital shear stressPresence of PD98059Synthetic phenotypeERK1/2 pathwayExtracellular signal-regulated proteinSMC proliferationSmooth muscle cell proliferationMuscle cell proliferationPathway inhibitor PD98059Cell proliferationExtracellular signal-regulated protein kinase 1/2 (ERK1/2) phosphorylationKinase 1/2 phosphorylationPresence of SB203580Bovine SMCsSMC phenotypeInhibitor PD98059Inhibitor SB203580Contractile phenotypePD98059Long-term culturePhenotypeCell nuclear antigenExpression of markersPathwaySB203580The role of STAT-3 in the mediation of smooth muscle cell response to cyclic strain
Kakisis JD, Pradhan S, Cordova A, Liapis CD, Sumpio BE. The role of STAT-3 in the mediation of smooth muscle cell response to cyclic strain. The International Journal Of Biochemistry & Cell Biology 2005, 37: 1396-1406. PMID: 15833272, DOI: 10.1016/j.biocel.2005.01.009.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCell ProliferationCells, CulturedDNA-Binding ProteinsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Myocytes, Smooth MusclePhosphorylationPyrazolesPyrimidinesRatsSerineSignal TransductionSrc-Family KinasesSTAT3 Transcription FactorStress, MechanicalTrans-ActivatorsTyrosineConceptsSerine phosphorylationTyrosine phosphorylationSmooth muscle cellsSTAT-3Vascular smooth muscle cellsSTAT-3 tyrosine phosphorylationSpecific inhibitorVascular cell morphologyExtracellular signal-regulated kinase 1/2Extracellular mechanical signalsSignal-regulated kinase 1/2Basal serine phosphorylationInhibition of SrcA7r5 smooth muscle cellsNegative feedback loopInhibition of ERK1/2Cells sensePhosphospecific antibodiesPhosphatidylinositol 3Signal transducerTranscription 3Kinase 2Kinase 1/2Smooth muscle cell responseBasal phosphorylationDifferential effects of orbital and laminar shear stress on endothelial cells
Dardik A, Chen L, Frattini J, Asada H, Aziz F, Kudo FA, Sumpio BE. Differential effects of orbital and laminar shear stress on endothelial cells. Journal Of Vascular Surgery 2005, 41: 869-880. PMID: 15886673, DOI: 10.1016/j.jvs.2005.01.020.Peer-Reviewed Original ResearchMAPKs (ERK½, p38) and AKT Can Be Phosphorylated by Shear Stress Independently of Platelet Endothelial Cell Adhesion Molecule-1 (CD31) in Vascular Endothelial Cells*
Sumpio BE, Yun S, Cordova AC, Haga M, Zhang J, Koh Y, Madri JA. MAPKs (ERK½, p38) and AKT Can Be Phosphorylated by Shear Stress Independently of Platelet Endothelial Cell Adhesion Molecule-1 (CD31) in Vascular Endothelial Cells*. Journal Of Biological Chemistry 2005, 280: 11185-11191. PMID: 15668248, DOI: 10.1074/jbc.m414631200.Peer-Reviewed Original ResearchAnimalsCattleCell CommunicationEndothelial CellsEnzyme ActivationHumansMechanoreceptorsMiceMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3P38 Mitogen-Activated Protein KinasesPhosphorylationPlatelet Endothelial Cell Adhesion Molecule-1Protein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktStress, MechanicalTyrosine
2004
Molecular and biological effects of hemodynamics on vascular cells.
Pradhan S, Sumpio B. Molecular and biological effects of hemodynamics on vascular cells. Frontiers In Bioscience-Landmark 2004, 9: 3276-85. PMID: 15353357, DOI: 10.2741/1480.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAtherosclerosisCell MovementCell ProliferationCells, CulturedCyclic AMPDiglyceridesDisease ProgressionEndothelial CellsExtracellular MatrixHemodynamicsHumansInositol 1,4,5-TrisphosphateIntercellular Adhesion Molecule-1Plasminogen ActivatorsProtein Kinase CRisk FactorsStress, MechanicalConceptsEndothelial cellsSignificant atherosclerotic plaqueSystemic risk factorsTissue plasminogen activatorCellular adhesion moleculesCurrent pertinent literatureCultured endothelial cellsRisk factorsSecond messenger systemsSignificant lesionsProtein kinase C pathwayICAM-1Atherosclerosis formationAtherosclerotic plaquesVascular anatomyKinase C pathwayNitric oxideVascular cellsPlasminogen activatorAdhesion moleculesBlood vesselsVascular biologyMessenger systemsVessel wallPertinent literatureCell signalling in vascular cells exposed to cyclic strain: the emerging role of protein phosphatases
Lee T, Sumpio BE. Cell signalling in vascular cells exposed to cyclic strain: the emerging role of protein phosphatases. Biotechnology And Applied Biochemistry 2004, 39: 129-139. PMID: 15032733, DOI: 10.1042/ba20030104.Peer-Reviewed Original ResearchConceptsPhosphorylation stateSerine/threonine residuesProtein phosphatase type 2AAbnormal phosphorylation stateCritical cellular functionsPhosphorylation of tyrosineRole of phosphatasesVascular cellsRole of proteinsThreonine residuesCellular functionsProtein kinaseIntracellular proteinsCellular growthHuman diseasesProteinPhosphataseType 2AEndothelial cellsCellsCardiac hypertrophyKinasePhosphorylationHaemodynamic forcesRole