2003
Thrombospondin-1 induces matrix metalloproteinase-2 activation in vascular smooth muscle cells1 1Competition of interest: none.
Lee T, Esemuede N, Sumpio BE, Gahtan V. Thrombospondin-1 induces matrix metalloproteinase-2 activation in vascular smooth muscle cells1 1Competition of interest: none. Journal Of Vascular Surgery 2003, 38: 147-154. PMID: 12844104, DOI: 10.1016/s0741-5214(02)75468-2.Peer-Reviewed Original ResearchConceptsThrombospondin-1TSP-1-induced VSMC migrationVSMC migrationCell migrationMatrix metalloproteinase-2MMP2 activationMatrix barrierSerum-free mediumNorthern blot analysisVascular smooth muscle cell migrationCellular processesExtracellular matrix glycoproteinMMP2 activityPosttranslational mechanismsMatrix metalloproteinase-2 activationMetalloproteinase-2 activationSmooth muscle cell migrationMuscle cell migrationInvasion assaysStrong binding activityBinding activityECM degradationMatrix glycoproteinRNA expressionBlot analysis
1998
Induction of interleukin (IL)‐1α and β gene expression in human keratinocytes exposed to repetitive strain: Their role in strain‐induced keratinocyte proliferation and morphological change
Takei T, Kito H, Du W, Mills I, Sumpio B. Induction of interleukin (IL)‐1α and β gene expression in human keratinocytes exposed to repetitive strain: Their role in strain‐induced keratinocyte proliferation and morphological change. Journal Of Cellular Biochemistry 1998, 69: 95-103. PMID: 9548558, DOI: 10.1002/(sici)1097-4644(19980501)69:2<95::aid-jcb1>3.0.co;2-p.Peer-Reviewed Original ResearchConceptsIL-1 antibodyIL-1 alphaIL-1Keratinocyte proliferationIL-1 mRNA expressionKeratinocyte biologyIL-1 gene expressionInduction of interleukinIL-1 betaIL-1 expressionMorphological changesΒ gene expressionAutocrine regulationMRNA expressionBeta mRNAGene expressionBasal rateBasic cellular propertiesHuman keratinocytesAntibodiesKeratinocyte functionRepetitive strainNorthern blot analysisBlot analysisProliferationCyclooxygenase Expression in Bovine Aortic Endothelial Cells Exposed to Cyclic Strain
Kito H, Yokoyama C, Inoue H, Tanabe T, Nakajima N, Sumpio B. Cyclooxygenase Expression in Bovine Aortic Endothelial Cells Exposed to Cyclic Strain. Endothelium 1998, 6: 107-112. PMID: 9930644, DOI: 10.3109/10623329809072197.Peer-Reviewed Original ResearchConceptsAortic endothelial cellsBovine aortic endothelial cellsEndothelial cellsInduction of COXCOX-1 expressionCOX-2 promoter activityExposure of ECsPromoter activityCycles/minCOX-2Cyclooxygenase expressionCOX-1Weak inducerMRNA expressionExtent of inductionNorthern blot analysisBlot analysisTransient transfection experimentsLipopolysaccharideMinimal effectExpressionInductionTransfection experimentsCyclic strainCells
1995
Cyclic strain upregulates nitric oxide synthase in cultured bovine aortic endothelial cells.
Awolesi MA, Sessa WC, Sumpio BE. Cyclic strain upregulates nitric oxide synthase in cultured bovine aortic endothelial cells. Journal Of Clinical Investigation 1995, 96: 1449-1454. PMID: 7544806, PMCID: PMC185768, DOI: 10.1172/jci118181.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthaseAortic endothelial cellsNitric oxide synthaseBovine aortic endothelial cellsCultured bovine aortic endothelial cellsEndothelial cellsOxide synthaseENOS transcriptsRegulation of eNOSENOS gene expressionBlot analysisHemodynamic forcesWestern blot analysisENOS levelsCycles/minImmunohistochemical stainingNitric oxideProtein levelsNorthern blot analysisNuclear runoff transcription assaysVivoCellsRunoff transcription assaysCytosolic RNAExpression