Hanieh Falahati, PhD
Associate Research ScientistAbout
Research
Publications
Featured Publications
Proximity proteomics of synaptopodin provides insight into the molecular composition of the spine apparatus of dendritic spines
Falahati H, Wu Y, Feuerer V, Simon HG, De Camilli P. Proximity proteomics of synaptopodin provides insight into the molecular composition of the spine apparatus of dendritic spines. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2203750119. PMID: 36215465, PMCID: PMC9586327, DOI: 10.1073/pnas.2203750119.Peer-Reviewed Original ResearchConceptsSpine apparatusDendritic spinesSubset of neuronsAxon initial segmentDendritic shaftsER cisternsNonneuronal cellsSynaptopodinSpineSmooth endoplasmic reticulumEndoplasmic reticulumCisternal organelleInitial segmentSpecific localizationCisternsBinding proteinPDLIM7Expression patternsSubsetProteinSmall subsetDiseaseNeuronsBrainFunctional partnershipTemperature-Induced uncoupling of cell cycle regulators
Falahati H, Hur W, Di Talia S, Wieschaus E. Temperature-Induced uncoupling of cell cycle regulators. Developmental Biology 2020, 470: 147-153. PMID: 33278404, PMCID: PMC8106975, DOI: 10.1016/j.ydbio.2020.11.010.Peer-Reviewed Original ResearchConceptsCell cycle eventsCycle eventsEarly cell cycle eventsMajor mitotic kinaseCell cycle stepsEarly Drosophila embryoCell cycle progressionCell cycle regulatorsDrosophila embryosMitotic kinasesChromosome condensationDifferent kinasesCdk1 activityEmbryo activationCycle progressionCycle regulatorsEnvironmental variabilityCell cycle transition timesPrometaphaseCDK1KinaseMitosisVivo biosensorsEmbryosThermodynamically driven assemblies and liquid–liquid phase separations in biology
Falahati H, Haji-Akbari A. Thermodynamically driven assemblies and liquid–liquid phase separations in biology. Soft Matter 2019, 15: 1135-1154. PMID: 30672955, DOI: 10.1039/c8sm02285b.Peer-Reviewed Original ResearchConceptsPhase separationBiological self-assembly processesSelf-assembly processLiquid-liquid phase separationUnderlying physical principlesDriven AssemblyIntermolecular interactionsPhysical originBiomolecular complexesMembraneless organellesMolecular simulationsPhysicsPhysical principlesStatistical physicsSeparationThermodynamic variablesExperimental verificationAssembly processSustenance of lifeThermodynamicsBiological implicationsAssemblyComputational techniquesSubcellular structuresEnergyIndependent active and thermodynamic processes govern the nucleolus assembly in vivo
Falahati H, Wieschaus E. Independent active and thermodynamic processes govern the nucleolus assembly in vivo. Proceedings Of The National Academy Of Sciences Of The United States Of America 2017, 114: 1335-1340. PMID: 28115706, PMCID: PMC5307486, DOI: 10.1073/pnas.1615395114.Peer-Reviewed Original ResearchNucleation by rRNA Dictates the Precision of Nucleolus Assembly
Falahati H, Pelham-Webb B, Blythe S, Wieschaus E. Nucleation by rRNA Dictates the Precision of Nucleolus Assembly. Current Biology 2016, 26: 277-285. PMID: 26776729, PMCID: PMC5866055, DOI: 10.1016/j.cub.2015.11.065.Peer-Reviewed Original ResearchConceptsMembrane-less organellesTranscription of rRNANucleolar proteinsCellular functionsNucleolus formationInitial assemblyD. melanogaster embryosDifferent cellular functionsSpecific cellular functionsWild-type embryosRole of rRNARibosomal biogenesisNucleolus assemblySuch organellesNucleoplasmic concentrationIntracellular compartmentsRRNAOrganellesSpatiotemporal precisionTranscriptionNucleoliEmbryosProteinAssemblyBiological systems
2024
Ectopic reconstitution of a spine-apparatus-like structure provides insight into mechanisms underlying its formation
Falahati H, Wu Y, Fang M, De Camilli P. Ectopic reconstitution of a spine-apparatus-like structure provides insight into mechanisms underlying its formation. Current Biology 2024 PMID: 39626668, DOI: 10.1016/j.cub.2024.11.010.Peer-Reviewed Original ResearchEndoplasmic reticulumSpine apparatusActin bundlesEndomembrane networkER sheetsConserved regionProtein synaptopodinCisternal organelleNon-neuronal cellsER cisternsOrganellesSynaptopodinProteinNeuronal dendritesNeuronal spinesAxon initial segmentFindings shed lightBiogenesisActinProtein matrixNarrow lumenReticulumMammalsInitial segmentMechanism
2021
Asynchronous release sites align with NMDA receptors in mouse hippocampal synapses
Li S, Raychaudhuri S, Lee SA, Brockmann MM, Wang J, Kusick G, Prater C, Syed S, Falahati H, Ramos R, Bartol TM, Hosy E, Watanabe S. Asynchronous release sites align with NMDA receptors in mouse hippocampal synapses. Nature Communications 2021, 12: 677. PMID: 33514725, PMCID: PMC7846561, DOI: 10.1038/s41467-021-21004-x.Peer-Reviewed Original Research
2020
Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells
Benedetti L, Marvin JS, Falahati H, Guillén-Samander A, Looger LL, De Camilli P. Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells. ELife 2020, 9: e63230. PMID: 33174843, PMCID: PMC7735757, DOI: 10.7554/elife.63230.Peer-Reviewed Original ResearchConceptsProtein fusion partnersSmall subcellular volumesSubcellular optogeneticsOrganelle contactsHomodimerization interfaceProtein modulesMammalian cellsBiological processesPhysiological processesSubcellular organellesLow temperature preincubationSimultaneous photoactivationFusion partnerCell preincubationWhole cellsSubcellular volumesConcatemerizationSpatial controlSpatiotemporal confinementCellsNeurosporaOrganellesHeterodimersVIVIDProtein
2016
Mitosis-associated repression in development
Esposito E, Lim B, Guessous G, Falahati H, Levine M. Mitosis-associated repression in development. Genes & Development 2016, 30: 1503-1508. PMID: 27401553, PMCID: PMC4949323, DOI: 10.1101/gad.281188.116.Peer-Reviewed Original ResearchConceptsSequence-specific repressorsEarly Drosophila embryoLive-imaging methodsMitotic silencingSnail repressionAnimal developmentSnail repressorDrosophila embryosNascent transcriptsNeurogenic ectodermTranscriptional repressionTarget enhancersPresumptive mesodermReporter geneRepressionRepressorMesodermTranscriptionMitosisEmbryosBroad patternsPervasive featureSilencingEctodermGenes
2015
Effect of sorbitol and glycerol on the stability of trypsin and difference between their stabilization effects in the various solvents
Pazhang M, Mehrnejad F, Pazhang Y, Falahati H, Chaparzadeh N. Effect of sorbitol and glycerol on the stability of trypsin and difference between their stabilization effects in the various solvents. Biotechnology And Applied Biochemistry 2015, 63: 206-213. PMID: 25757511, DOI: 10.1002/bab.1366.Peer-Reviewed Original Research
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- Spine Apparatus (orange) is a specialization of neuronal endoplasmic reticulum, which is located at dendritic spines.