Featured Publications
Proximity proteomics of synaptopodin provides insight into the molecular composition of the spine apparatus of dendritic spines
Falahati H, Wu Y, Feuerer V, Simon HG, De Camilli P. Proximity proteomics of synaptopodin provides insight into the molecular composition of the spine apparatus of dendritic spines. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2203750119. PMID: 36215465, PMCID: PMC9586327, DOI: 10.1073/pnas.2203750119.Peer-Reviewed Original ResearchConceptsSpine apparatusDendritic spinesSubset of neuronsAxon initial segmentDendritic shaftsER cisternsNonneuronal cellsSynaptopodinSpineSmooth endoplasmic reticulumEndoplasmic reticulumCisternal organelleInitial segmentSpecific localizationCisternsBinding proteinPDLIM7Expression patternsSubsetProteinSmall subsetDiseaseNeuronsBrainFunctional partnershipTemperature-Induced uncoupling of cell cycle regulators
Falahati H, Hur W, Di Talia S, Wieschaus E. Temperature-Induced uncoupling of cell cycle regulators. Developmental Biology 2020, 470: 147-153. PMID: 33278404, PMCID: PMC8106975, DOI: 10.1016/j.ydbio.2020.11.010.Peer-Reviewed Original ResearchConceptsCell cycle eventsCycle eventsEarly cell cycle eventsMajor mitotic kinaseCell cycle stepsEarly Drosophila embryoCell cycle progressionCell cycle regulatorsDrosophila embryosMitotic kinasesChromosome condensationDifferent kinasesCdk1 activityEmbryo activationCycle progressionCycle regulatorsEnvironmental variabilityCell cycle transition timesPrometaphaseCDK1KinaseMitosisVivo biosensorsEmbryosThermodynamically driven assemblies and liquid–liquid phase separations in biology
Falahati H, Haji-Akbari A. Thermodynamically driven assemblies and liquid–liquid phase separations in biology. Soft Matter 2019, 15: 1135-1154. PMID: 30672955, DOI: 10.1039/c8sm02285b.Peer-Reviewed Original ResearchConceptsPhase separationBiological self-assembly processesSelf-assembly processLiquid-liquid phase separationUnderlying physical principlesDriven AssemblyIntermolecular interactionsPhysical originBiomolecular complexesMembraneless organellesMolecular simulationsPhysicsPhysical principlesStatistical physicsSeparationThermodynamic variablesExperimental verificationAssembly processSustenance of lifeThermodynamicsBiological implicationsAssemblyComputational techniquesSubcellular structuresEnergyIndependent active and thermodynamic processes govern the nucleolus assembly in vivo
Falahati H, Wieschaus E. Independent active and thermodynamic processes govern the nucleolus assembly in vivo. Proceedings Of The National Academy Of Sciences Of The United States Of America 2017, 114: 1335-1340. PMID: 28115706, PMCID: PMC5307486, DOI: 10.1073/pnas.1615395114.Peer-Reviewed Original ResearchNucleation by rRNA Dictates the Precision of Nucleolus Assembly
Falahati H, Pelham-Webb B, Blythe S, Wieschaus E. Nucleation by rRNA Dictates the Precision of Nucleolus Assembly. Current Biology 2016, 26: 277-285. PMID: 26776729, PMCID: PMC5866055, DOI: 10.1016/j.cub.2015.11.065.Peer-Reviewed Original ResearchConceptsMembrane-less organellesTranscription of rRNANucleolar proteinsCellular functionsNucleolus formationInitial assemblyD. melanogaster embryosDifferent cellular functionsSpecific cellular functionsWild-type embryosRole of rRNARibosomal biogenesisNucleolus assemblySuch organellesNucleoplasmic concentrationIntracellular compartmentsRRNAOrganellesSpatiotemporal precisionTranscriptionNucleoliEmbryosProteinAssemblyBiological systems
2021
Asynchronous release sites align with NMDA receptors in mouse hippocampal synapses
Li S, Raychaudhuri S, Lee SA, Brockmann MM, Wang J, Kusick G, Prater C, Syed S, Falahati H, Ramos R, Bartol TM, Hosy E, Watanabe S. Asynchronous release sites align with NMDA receptors in mouse hippocampal synapses. Nature Communications 2021, 12: 677. PMID: 33514725, PMCID: PMC7846561, DOI: 10.1038/s41467-021-21004-x.Peer-Reviewed Original Research
2020
Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells
Benedetti L, Marvin JS, Falahati H, Guillén-Samander A, Looger LL, De Camilli P. Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells. ELife 2020, 9: e63230. PMID: 33174843, PMCID: PMC7735757, DOI: 10.7554/elife.63230.Peer-Reviewed Original ResearchConceptsProtein fusion partnersSmall subcellular volumesSubcellular optogeneticsOrganelle contactsHomodimerization interfaceProtein modulesMammalian cellsBiological processesPhysiological processesSubcellular organellesLow temperature preincubationSimultaneous photoactivationFusion partnerCell preincubationWhole cellsSubcellular volumesConcatemerizationSpatial controlSpatiotemporal confinementCellsNeurosporaOrganellesHeterodimersVIVIDProtein
2016
Mitosis-associated repression in development
Esposito E, Lim B, Guessous G, Falahati H, Levine M. Mitosis-associated repression in development. Genes & Development 2016, 30: 1503-1508. PMID: 27401553, PMCID: PMC4949323, DOI: 10.1101/gad.281188.116.Peer-Reviewed Original ResearchConceptsSequence-specific repressorsEarly Drosophila embryoLive-imaging methodsMitotic silencingSnail repressionAnimal developmentSnail repressorDrosophila embryosNascent transcriptsNeurogenic ectodermTranscriptional repressionTarget enhancersPresumptive mesodermReporter geneRepressionRepressorMesodermTranscriptionMitosisEmbryosBroad patternsPervasive featureSilencingEctodermGenes
2015
Effect of sorbitol and glycerol on the stability of trypsin and difference between their stabilization effects in the various solvents
Pazhang M, Mehrnejad F, Pazhang Y, Falahati H, Chaparzadeh N. Effect of sorbitol and glycerol on the stability of trypsin and difference between their stabilization effects in the various solvents. Biotechnology And Applied Biochemistry 2015, 63: 206-213. PMID: 25757511, DOI: 10.1002/bab.1366.Peer-Reviewed Original ResearchBiochemical Characterization and Computational Identification of Mycobacterium tuberculosis Pyrazinamidase in Some Pyrazinamide-Resistant Isolates of Iran
Doustdar F, Pazhang M, Mehrnejad F, Safarzadeh M, Rabiei D, Chaparzadeh N, Falahati H, Mir-Derikvand M. Biochemical Characterization and Computational Identification of Mycobacterium tuberculosis Pyrazinamidase in Some Pyrazinamide-Resistant Isolates of Iran. The Protein Journal 2015, 34: 181-192. PMID: 25972249, DOI: 10.1007/s10930-015-9610-5.Peer-Reviewed Original Research
2013
Transmitting the allosteric signal in methylglyoxal synthase
Falahati H, Pazhang M, Zareian S, Ghaemi N, Rofougaran R, Hofer A, Rezaie AR, Khajeh K. Transmitting the allosteric signal in methylglyoxal synthase. Protein Engineering Design And Selection 2013, 26: 445-452. PMID: 23592737, DOI: 10.1093/protein/gzt014.Peer-Reviewed Original ResearchConceptsAllosteric signalMethylglyoxal synthaseEnzyme methylglyoxal synthaseSite of phosphateActive site amino acidsSite-directed mutagenesisSite amino acidsAllosteric networkDynamic proteinsTerminal tailNew salt bridgesSer-55Gly-56Allosteric mechanismActive siteAsp-10Val-101Arg-97AllosteryThermus spAmino acidsPhosphoryl groupHomotropic allosterySalt bridgeModel system
2011
Mesophilic alcohol dehydrogenase behavior in imidazolium based ionic liquids
Dabirmanesh B, Khajeh K, Akbari J, Falahati H, Daneshjoo S, Heydari A. Mesophilic alcohol dehydrogenase behavior in imidazolium based ionic liquids. Journal Of Molecular Liquids 2011, 161: 139-143. DOI: 10.1016/j.molliq.2011.05.007.Peer-Reviewed Original ResearchIonic liquidsWater-miscible ionic liquidMiscible ionic liquidsAqueous IL mixturesIonic liquid concentrationAssociated conformational changesIL cationsIL mixturesOrganic solventsCatalyzed reactionsBiocatalytic processesLiquid concentrationLiquidConformational changesFluorescence techniqueStabilityImidazoliumWide applicationAnionsAlcohol dehydrogenaseSolventCationsILsReactionConcentrationMesophilic alcohol dehydrogenase behavior in imidazolium based ionic liquids
Dabirmanesh, Bahareh, Khosro Khajeh, Jafar Akbari, Hanieh Falahati, Somayeh Daneshjoo, and Akbar Heydari. Journal of Molecular Liquids 161, no. 3 (2011): 139-143.Peer-Reviewed Original Research
2010
Cloning, Expression, and Characterization of a Novel Methylglyoxal Synthase from Thermus sp. Strain GH5
Pazhang M, Khajeh K, Asghari SM, Falahati H, Naderi-Manesh H. Cloning, Expression, and Characterization of a Novel Methylglyoxal Synthase from Thermus sp. Strain GH5. Applied Biochemistry And Biotechnology 2010, 162: 1519-1528. PMID: 20419481, DOI: 10.1007/s12010-010-8933-0.Peer-Reviewed Original ResearchConceptsMethylglyoxal synthaseThermus spAmino acid sequenceGel filtration experimentsAcid sequenceHexameric enzymeAllosteric inhibitorsQuaternary structureEnzyme subunitMolecular massAmino acidsArg-150GH5Q-SepharoseGenesTMGSSpEnzymeSynthasePresence of phosphateOptimum activityHill coefficientCloningSubunitsPolypeptide
2009
Effects of ionic liquids on the kinetic behavior of thermophilic alcohol dehydrogenase and its mesophilic counterpart
Dabirmanesh B, Falahati H, Khajeh K, Akbari J, Heydari A. Effects of ionic liquids on the kinetic behavior of thermophilic alcohol dehydrogenase and its mesophilic counterpart. New Biotechnology 2009, 25: s143. DOI: 10.1016/j.nbt.2009.06.470.Peer-Reviewed Original Research