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INFORMATION FOR

Services

Biological electron microscopy services:

· Cell and tissue sample processing, embedding in resin for TEM

· Ultramicrotomy of plastic sections

· Cryo-sectioning and immuno-gold labeling (Tokuyasu method)

· Immuno-electron microscopy

· Transmission electron microscopy and digital imaging

· Scanning electron microscopy

· Electron tomography for resin embedded samples (single/dual tilt series)

· Focused ion beam SEM sample preparation and 3D data collection (FIB-SEM)

· Training on operating various electron microscopes and sample prep instrument

· Consultation and assistance in experimental design, grant application and publication

Cryo electron microscopy services:

For independent users

The EM facility recommends that a lab member be assigned to a cryo EM project due to its complex nature and long-term commitment. The point person will be trained and tested on the Tecnai T12 TEM and expected to operate independently for at least 20 hours. Subsequent training on cryo-sample preparation should enable the user to perform cryoEM experiments on their own. With practice, users at this level are able to operate the microscopes and image samples independently. Once they have demonstrated sufficient expertise on the equipment, they are authorized to work during off hours or on weekends.

For collaborating users

Users have discussed the project with the EM Facility and established a system of collaboration. There will be a trial period in which the feasibility of the project will be evaluated. If the project is deemed reasonable, the staff will provide substantial guidance and assistance to the project which can include data collection and image analysis.

Use of Glacios cryo TEM

Currently we offer four days of screening/data collection and one day of screening only per week. Users have choice for either option. Signing up a whole-day slot has advantage of over-night data collection without additional charge. As of now, we only offer the assisted use from Monday to Friday, but expect to provide unassisted use in near future to qualified users as well.

Anyone who are interested please contact the facility for arrangement. It would be first come first serve, the screening day in the week will be determined on weekly base depending on user requests and the microscope availability.