2012
NKCC1 Knockdown Decreases Neuron Production through GABAA-Regulated Neural Progenitor Proliferation and Delays Dendrite Development
Young SZ, Taylor MM, Wu S, Ikeda-Matsuo Y, Kubera C, Bordey A. NKCC1 Knockdown Decreases Neuron Production through GABAA-Regulated Neural Progenitor Proliferation and Delays Dendrite Development. Journal Of Neuroscience 2012, 32: 13630-13638. PMID: 23015452, PMCID: PMC3478384, DOI: 10.1523/jneurosci.2864-12.2012.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnalysis of VarianceAnimalsAnimals, NewbornCalciumCell CountCell DifferentiationCell ProliferationCells, CulturedCerebral VentriclesDendritesEgtazic AcidElectroporationFemaleGABA ModulatorsGABA-A Receptor AgonistsGreen Fluorescent ProteinsIn Vitro TechniquesKi-67 AntigenLuminescent ProteinsMaleMiceMuscimolNeural Stem CellsNeuronsOlfactory BulbPatch-Clamp TechniquesPentobarbitalReceptors, GABA-ARNA, Small InterferingSodium-Potassium-Chloride SymportersSolute Carrier Family 12, Member 2SOXB1 Transcription FactorsTransfectionConceptsNPC proliferationDecreased neuronal densityTotal dendritic lengthNeonatal subventricular zoneNeural stem cell proliferationNeural progenitor cell developmentNeural progenitor proliferationShort hairpin RNADendritic complexityDendritic lengthNeuronal densityNewborn neuronsDendritic arborizationNeuron densityDendritic developmentSubventricular zoneNeuron productionCalcium responseSynaptic integrationNKCC1 knockdownPentobarbital effectsAllosteric agonistDendritic treeProgenitor cell developmentCotransporter NKCC1Preparation of acute subventricular zone slices for calcium imaging.
Lacar B, Young SZ, Platel JC, Bordey A. Preparation of acute subventricular zone slices for calcium imaging. Journal Of Visualized Experiments 2012, e4071. PMID: 23023088, PMCID: PMC3490239, DOI: 10.3791/4071.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsCell typesSVZ cellsProgenitor cellsNeurogenic zonesSubventricular zoneFluo-4 AM dyeNeural progenitor cellsIntermediate progenitor cellsExtracellular signalsDiffusible signalsIntercellular signalingSVZ cell typesTime-lapse moviesCalcium indicator dyeCalcium activityIndividual cellsRostral-caudal axisNeuroblast migrationThree-dimensional arrangementAdult hippocampal subgranular zonePostnatal brainAdhesion moleculesHippocampal subgranular zoneMature astrocytesNeuroblasts
2011
Gap junction‐mediated calcium waves define communication networks among murine postnatal neural progenitor cells
Lacar B, Young SZ, Platel J, Bordey A. Gap junction‐mediated calcium waves define communication networks among murine postnatal neural progenitor cells. European Journal Of Neuroscience 2011, 34: 1895-1905. PMID: 22098557, PMCID: PMC3237798, DOI: 10.1111/j.1460-9568.2011.07901.x.Peer-Reviewed Original ResearchConceptsNeural progenitor cellsNeurogenic nicheB2 cellsBlood vesselsCalcium wavesPurinergic receptor blockerProgenitor cellsPostnatal neurogenic nichesFunctional couplingPostnatal neural progenitor cellsGap junction blockersGap junction protein connexin 43Neonatal electroporationNiche astrocytesReceptor blockersProtein connexin 43Intercellular calcium wavesSubventricular zoneCells persistJunction blockersB1 cellsB cellsDye couplingDistinct entityConnexin 43
2010
Prostaglandin E2 induces glutamate release from subventricular zone astrocytes.
Dave KA, Platel JC, Huang F, Tian D, Stamboulian-Platel S, Bordey A. Prostaglandin E2 induces glutamate release from subventricular zone astrocytes. Neuron Glia Biology 2010, 6: 201-7. PMID: 21211110, DOI: 10.1017/s1740925x10000244.Peer-Reviewed Original ResearchConceptsAmbient glutamate levelsProstaglandin E2Subventricular zoneGlutamate releaseAstrocyte-like cellsGlutamate levelsGramicidin-perforated patch-clamp techniquesIntracellular Ca2Application of PGE2Aspartate receptor channelsPatch-clamp techniqueLateral ventricleSVZ cellsPGE2 releaseChoroid plexusMature astrocytesNeuroblast survivalEnzyme immunoassayReceptor channelsAstrocytesE2Ca2CellsReleaseLesser extent
2009
GABA increases Ca2+ in cerebellar granule cell precursors via depolarization: Implications for proliferation
Dave KA, Bordey A. GABA increases Ca2+ in cerebellar granule cell precursors via depolarization: Implications for proliferation. IUBMB Life 2009, 61: 496-503. PMID: 19391160, PMCID: PMC2675662, DOI: 10.1002/iub.185.Peer-Reviewed Original ResearchConceptsGamma-aminobutyric acidRole of GABAVertebrate central nervous systemComplement of receptorsCerebellar granule cell precursorsCell precursorsGranule cell precursorsDevelopmental processesMetabotropic glutamate receptorsCentral nervous systemCell proliferationAdult mammalsNovel targetAmino acid glutamatePharmacological regulationCurrent knowledgeGlutamate receptorsNeural cancersNervous systemCerebellar tumorsCalcium increaseReceptorsProliferationMammalsNovel data
2004
Glial Glutamate Transporters Limit Spillover Activation of Presynaptic NMDA Receptors and Influence Synaptic Inhibition of Purkinje Neurons
Huang H, Bordey A. Glial Glutamate Transporters Limit Spillover Activation of Presynaptic NMDA Receptors and Influence Synaptic Inhibition of Purkinje Neurons. Journal Of Neuroscience 2004, 24: 5659-5669. PMID: 15215288, PMCID: PMC6729224, DOI: 10.1523/jneurosci.1338-04.2004.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAmino Acid Transport System X-AGAnimalsAntiportersCalciumCerebellumCystineEvoked PotentialsGamma-Aminobutyric AcidGlutamic AcidIn Vitro TechniquesMiceNerve FibersNeural InhibitionNeurogliaPatch-Clamp TechniquesPurkinje CellsReceptors, N-Methyl-D-AspartateReceptors, PresynapticRyanodineSynaptic TransmissionConceptsGlial glutamate transportersMiniature IPSCsPurkinje neuronsGlutamate transportersSpontaneous IPSCsSynaptic inhibitionInhibitory synapsesNMDAR activationWhole-cell patch-clamp recordingsPresynaptic NMDA receptorsPaired-pulse ratioMouse cerebellar slicesNMDA receptor activationPresynaptic NMDA receptor activationPatch-clamp recordingsBergmann glial cellsBeta-benzyloxyaspartic acidAdjacent Purkinje neuronsCystine-glutamate antiporterMIPSC frequencyGABAergic terminalsGABAergic synapsesGlial cellsNMDA receptorsExcitatory synapses
2003
Chemokine modulation of high‐conductance Ca2+‐sensitive K+ currents in microglia from human hippocampi
Bordey A, Spencer DD. Chemokine modulation of high‐conductance Ca2+‐sensitive K+ currents in microglia from human hippocampi. European Journal Of Neuroscience 2003, 18: 2893-2898. PMID: 14656339, DOI: 10.1111/j.1460-9568.2003.03021.x.Peer-Reviewed Original ResearchConceptsHigh-conductance Ca2MIP1-alphaAcute pathological processesNon-activated microgliaSensitive outward currentsPharmaco-resistant epilepsyPatch-clamp recordingsOutward current amplitudeHigh input resistanceChemokine modulationHippocampal slicesInjury siteBrain slicesChronic pathologiesMicrogliaEpileptic patientsAmeboid morphologyIntracellular Ca2Human hippocampusOutward currentsMigratory effectsInput resistancePositive labelingPathological processesMode of actionBiophysical Properties and Ionic Signature of Neuronal Progenitors of the Postnatal Subventricular Zone In Situ
Wang DD, Krueger DD, Bordey A. Biophysical Properties and Ionic Signature of Neuronal Progenitors of the Postnatal Subventricular Zone In Situ. Journal Of Neurophysiology 2003, 90: 2291-2302. PMID: 12801891, DOI: 10.1152/jn.01116.2002.Peer-Reviewed Original ResearchConceptsRostral migratory streamSubventricular zonePassive membrane propertiesNeuronal progenitorsCell-attached patch-clamp recordingsNeuron-specific class IIIPostnatal mammalian brainPostnatal day 15Patch-clamp recordingsMigration of progenitorsPostnatal subventricular zoneHigh input resistanceCell-attached patchesBrain slicesOlfactory bulbMature neuronsStrong immunoreactivityTransient outwardDay 15KDR currentElectrophysiological propertiesAction potentialsMammalian brainMigratory streamClass III
2000
Muscarinic Activation of BK Channels Induces Membrane Oscillations in Glioma Cells and Leads to Inhibition of Cell Migration
Bordey A, Sontheimer H, Trouslard J. Muscarinic Activation of BK Channels Induces Membrane Oscillations in Glioma Cells and Leads to Inhibition of Cell Migration. The Journal Of Membrane Biology 2000, 176: 31-40. PMID: 10882426, DOI: 10.1007/s00232001073.Peer-Reviewed Original ResearchConceptsPatch-clamp recordingsGlioma cellsAstrocytoma cellsCell migrationCultured human astrocytoma cellsIntracellular free calcium concentrationACh receptor agonistsFree calcium concentrationHuman astrocytoma cellsRelease of Ca2Glioma cell migrationMuscarinic activationMuscarinic antagonistCerebral tumorsMuscarinic receptorsReceptor agonistBath applicationActivate Ca2Intracellular injectionCerebrospinal fluidACh applicationAcetylcholineBiphasic increaseIntracellular storesExternal calcium
1998
Passive Glial Cells, Fact or Artifact?
Bordey A, Sontheimer H. Passive Glial Cells, Fact or Artifact? The Journal Of Membrane Biology 1998, 166: 213-222. PMID: 9843595, DOI: 10.1007/s002329900463.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsVoltage-activated currentsPassive astrocytesVoltage-dependent outwardCell accessAcute tissue slicesBath Ca2Glial cellsRat hippocampusSlice recordingsCultured astrocytesAstrocytesPipette solutionEffect of Ca2Tissue slicesMembrane capacitanceSuch cellsSubpopulationsCellsTransient maskingConcentration of Ca2Ca2SuperfusionHippocampusDose
1994
Mobilization of intracellular calcium by substance p in a human astrocytoma cell line (U‐373 MG)
Bordey A, Feltz P, Trouslard J. Mobilization of intracellular calcium by substance p in a human astrocytoma cell line (U‐373 MG). Glia 1994, 11: 277-283. PMID: 7525479, DOI: 10.1002/glia.440110309.Peer-Reviewed Original ResearchConceptsSubstance PReceptor agonistConcentrations of SPEffects of SPIntracellular free calcium concentrationSelective receptor agonistsNK1 receptor agonistNM substance PIntracellular calcium poolsFura-2 acetoxymethylesterHuman astrocytoma cell lineStable basal levelExtracellular calcium ionsFree calcium concentrationAstrocytoma cell linesHuman astrocytoma cellsPertussis treatmentNK1 receptorsIntracellular calciumCalcium poolMaximal effectCytosolic calciumAstrocytoma cellsBasal levelsCalcium concentrationSR 140333, a Novel, Selective, and Potent Nonpeptide Antagonist of the NK1 Tachykinin Receptor: Characterization on the U373MG Cell Line
Oury‐Donat F, Lefevre IA, Thurneyssen O, Gauthier T, Bordey A, Feltz P, Emonds‐Alt X, Le Fur G, Soubrie P. SR 140333, a Novel, Selective, and Potent Nonpeptide Antagonist of the NK1 Tachykinin Receptor: Characterization on the U373MG Cell Line. Journal Of Neurochemistry 1994, 62: 1399-1407. PMID: 7510780, DOI: 10.1046/j.1471-4159.1994.62041399.x.Peer-Reviewed Original ResearchMeSH KeywordsAstrocytomaBinding, CompetitiveCalciumElectric ConductivityGenes, fosHumansInositol PhosphatesIodine RadioisotopesNeurokinin-1 Receptor AntagonistsPeptide FragmentsPiperidinesPotassiumPyrrolidonecarboxylic AcidQuinuclidinesReceptors, Neurokinin-1StereoisomerismSubstance PSuccinimidesTaurineTranscription, GeneticTumor Cells, CulturedConceptsSR 140333Substance PNK1 tachykinin receptor antagonistNK1 receptor activationHunter substance PNK1-selective agonistTachykinin receptor antagonistsInositol monophosphate formationNK1 tachykinin receptorsPotent nonpeptide antagonistHuman astrocytoma cell lineCell linesAstrocytoma cell linesPatch-clamp experimentsReceptor antagonistFormation of inositolTachykinin receptorsNK1 agonistU373MG cell linesCalcium mobilizationNonpeptide antagonistsRadioligand bindingReceptor activationOutward currentsMonophosphate formation