2017
Determining mRNA Decay Rates Using RNA Approach to Equilibrium Sequencing (RATE-Seq)
Abdul-Rahman F, Gresham D. Determining mRNA Decay Rates Using RNA Approach to Equilibrium Sequencing (RATE-Seq). Methods In Molecular Biology 2017, 1720: 15-24. PMID: 29236248, DOI: 10.1007/978-1-4939-7540-2_2.Peer-Reviewed Original ResearchConceptsNewly transcribed RNAPopulation of RNAsMRNA decay ratesRNA spike-insRNA degradation ratesStreptavidin magnetic beadsBiotinylated RNARNA speciesUnlabeled RNARNAseq librariesSynthesized RNASpike-insRNA approachBiotinylated moleculesRNAReactive thiol groupsCultures of cellsMagnetic beadsDegradation rateDegradation rate constantLabeled populationThiol groupsTranscriptionRNAseqMultiple time points
1999
Neuronal protection and destruction by NO
Lipton S. Neuronal protection and destruction by NO. Cell Death & Differentiation 1999, 6: 943-951. PMID: 10556970, DOI: 10.1038/sj.cdd.4400580.Peer-Reviewed Original ResearchConceptsS-nitrosylationCysteine residuesCaspase enzyme activityNeuronal apoptotic pathwayProtein functionCritical thiol groupsApoptotic pathwayMolecular switchReactive thiol groupsCritical thiolsN-methyl-D-aspartate receptor activityDisulfide bondsRedox stateThiol groupsDeath of neuronsSpeciesDifferent redox statesEnzyme activityDistinct chemical reactivityCaspasesPhysiological conditionsBiological activityActive siteResiduesReceptor activity
1998
Chapter 6 Redox modulation of the NMDA receptor by NO-related species
Lipton S, Rayudu P, Choi Y, Sucher N, Chen H. Chapter 6 Redox modulation of the NMDA receptor by NO-related species. Progress In Brain Research 1998, 118: 73-82. PMID: 9932435, DOI: 10.1016/s0079-6123(08)63201-x.Peer-Reviewed Original ResearchConceptsChemical reactionsMechanism of reactionRedox stateReactive thiol groupsCatalytic amountFurther oxidationThiol groupsElectron acceptorPreferred reactionReceptor sulfhydryl groupsChemical evidenceSinglet stateNO groupBiological activityMolecular switchReactionThiolsDisulfide bondsSulfhydryl groupsCritical thiolsO2NOS-nitrosylationRedox modulationBonds
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