Human Quiescin-Sulfhydryl Oxidase, QSOX1: Probing Internal Redox Steps by Mutagenesis
Heckler E, Alon A, Fass D, Thorpe C. Human Quiescin-Sulfhydryl Oxidase, QSOX1: Probing Internal Redox Steps by Mutagenesis. Biochemistry 2008, 47: 4955-4963. PMID: 18393449, PMCID: PMC3542536, DOI: 10.1021/bi702522q.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsAmino Acid SequenceAnimalsCatalysisCattleCysteineDisulfidesEgg ProteinsGene Expression Regulation, BacterialHumansMilk ProteinsMolecular Sequence DataMutagenesisOxidation-ReductionOxidoreductases Acting on Sulfur Group DonorsProtein FoldingProtein Structure, TertiaryRecombinant ProteinsSequence Analysis, ProteinSequence Homology, Amino AcidThioredoxinsConceptsQuiescin sulfhydryl oxidaseThioredoxin domainInserts disulfide bondsRecombinant expression systemProximal disulfideAvian enzymeEnzymological characterizationHeterologous expressionEnzyme familyErv/ALR domainDimer interfaceExpression systemPolypeptide chainOxygen to hydrogen peroxideReducing equivalentsCysteine residuesMechanistic dissectionFlavin ringCysteine mutantsRNaseActivity assayAvian eggsReduction of oxygen to hydrogen peroxideDisulfide bondsRedox steps
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