2020
Whole-Cell Photobleaching Reveals Time-Dependent Compartmentalization of Soluble Proteins by the Axon Initial Segment
Nicholson L, Gervasi N, Falières T, Leroy A, Miremont D, Zala D, Hanus C. Whole-Cell Photobleaching Reveals Time-Dependent Compartmentalization of Soluble Proteins by the Axon Initial Segment. Frontiers In Cellular Neuroscience 2020, 14: 180. PMID: 32754013, PMCID: PMC7366827, DOI: 10.3389/fncel.2020.00180.Peer-Reviewed Original ResearchSoluble proteinAxon initial segmentCytoplasmic soluble proteinsForm of compartmentalizationIntact F-actinSuper-resolution fluorescence imagingSpectrin cytoskeletonSpecific proteinsF-actinProtein accumulationAxonal compartmentProtein exchangeProteinCompartmentalizationSomatodendritic compartmentNeuronal morphologyInitial segmentImportant insightsUltrastructureCompartmentsFluorescence imagingPhotobleachingCytoskeletonSignalingDifferentiation
2015
Chapter 11 Analysis of soluble protein entry into primary cilia using semipermeabilized cells
Breslow DK, Nachury MV. Chapter 11 Analysis of soluble protein entry into primary cilia using semipermeabilized cells. Methods In Cell Biology 2015, 127: 203-221. PMID: 25837393, PMCID: PMC4797650, DOI: 10.1016/bs.mcb.2014.12.006.BooksConceptsSemipermeabilized cellsProtein entriesPrimary ciliaCiliary diffusion barrierNuclear pore complexPrimary cilia functionPore complexMammalian cellsSignal transductionSpecialized compartmentsCilia functionPlasma membraneCiliary membraneIntact cellsExperimental perturbationsCell surfaceProtein exchangeCiliaVitro systemAxon initial segmentMechanistic analysisChapter 11 AnalysisUnique resourceCapture assayCells
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