1999
Functional partnership between amphiphysin and dynamin in clathrin-mediated endocytosis
Takei K, Slepnev V, Haucke V, De Camilli P. Functional partnership between amphiphysin and dynamin in clathrin-mediated endocytosis. Nature Cell Biology 1999, 1: 33-39. PMID: 10559861, DOI: 10.1038/9004.Peer-Reviewed Original ResearchConceptsDynamin 1Functional partnershipCell-free systemAmphiphysin 1Clathrin coatDynaminAmphiphysinRing-like structurePresence of GTPSynaptic vesiclesEndocytosisNarrow tubulesLipid bilayersMorphological evidenceBilayer curvatureVesiclesSpherical liposomesPotential functionClathrinGTPDirect morphological evidenceProteinAssembles
1997
Gi regulation of secretory vesicle swelling examined by atomic force microscopy
Jena B, Schneider S, Geibel J, Webster P, Oberleithner H, Sritharan K. Gi regulation of secretory vesicle swelling examined by atomic force microscopy. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 13317-13322. PMID: 9371843, PMCID: PMC24306, DOI: 10.1073/pnas.94.24.13317.Peer-Reviewed Original ResearchConceptsSecretory vesicle membraneVesicle swellingSecretory vesiclesPlasma membraneVesicle fusionVesicle membraneSecretory vesicle swellingSecretory vesicle fusionConfocal microscopyZymogen granulesCell plasma membraneVesicle volumeHeterotrimeric guanineHeterotrimeric GTPZymogen granule sizeGTPase activityAlpha subunitMolecular mechanismsRegulatory rolePresence of GTPExocytotic processMastoparan analogsProteinGi proteinsMas7
1994
GTP-dependent binding of the antiproliferative agent didemnin to elongation factor 1 alpha.
Crews CM, Collins JL, Lane WS, Snapper ML, Schreiber SL. GTP-dependent binding of the antiproliferative agent didemnin to elongation factor 1 alpha. Journal Of Biological Chemistry 1994, 269: 15411-15414. PMID: 8195179, DOI: 10.1016/s0021-9258(17)40692-2.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAnti-Infective AgentsCarrier ProteinsCattleChromatography, AffinityDepsipeptidesGuanosine TriphosphateHumansIndicators and ReagentsKineticsMolecular Sequence DataPeptide Elongation Factor 1Peptide Elongation FactorsPeptides, CyclicProtein Synthesis InhibitorsSequence Homology, Amino AcidConceptsEF-1 alphaPotential antineoplastic drugElongation factor 1 alphaDidemnin BProtein synthesisAntiproliferative activityG1 cell cycle progressionGTP-dependent bindingFactor 1 alphaClinical trialsCell cycle progressionImmunosuppressive activityAntineoplastic drugsPeptide sequence analysisElongation factorMode of actionUndefined mechanismPresence of GTPGTPase activityCycle progressionNanomolar concentrationsSequence analysisAlphaMarine natural productsIntracellular targets
1991
Acute electrophysiological responses of bradykinin‐stimulated human fibroblasts.
Estacion M. Acute electrophysiological responses of bradykinin‐stimulated human fibroblasts. The Journal Of Physiology 1991, 436: 603-620. PMID: 1648132, PMCID: PMC1181524, DOI: 10.1113/jphysiol.1991.sp018569.Peer-Reviewed Original ResearchConceptsFura-2 fluorescencePipette solutionIon substitution experimentsGTP gamma SReversal potentialBradykinin stimulationPresence of GTPHuman dermal fibroblastsGamma SPatch-clamp techniqueNon-specific cation channelsBradykinin additionDermal fibroblastsIntracellular calciumLittle voltage dependencePatch-clamped cellsTail current analysisAcute responseInward currentsCalcium ionophoreOutward currentsNear baselineCation channelsElectrophysiological responsesBradykinin
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