2023
HEXIM1 is an essential transcription regulator during human erythropoiesis
Lv X, Murphy K, Murphy Z, Getman M, Rahman N, Nakamura Y, Blanc L, Gallagher P, Palis J, Mohandas N, Steiner L. HEXIM1 is an essential transcription regulator during human erythropoiesis. Blood 2023, 142: 2198-2215. PMID: 37738561, PMCID: PMC10733840, DOI: 10.1182/blood.2022019495.Peer-Reviewed Original ResearchConceptsFetal globin expressionGene expressionGlobin expressionCycle progressionErythroid gene expressionBeta-globinBeta-globin locusGenome-wide profilingRNA polymerase II activityLong non-coding RNANon-coding RNAErythroid proliferationPolymerase II activityCell cycle progressionEssential transcription regulatorRNAPII activityRNAPII occupancyGlobin locusTranscription machineryTranscription regulatorsFetal globinRNAPIIFetal gene expressionHEXIM1Human erythropoiesis
2021
Regulation of RNA polymerase II activity is essential for terminal erythroid maturation
Murphy ZC, Murphy K, Myers J, Getman M, Couch T, Schulz VP, Lezon-Geyda K, Palumbo C, Yan H, Mohandas N, Gallagher PG, Steiner LA. Regulation of RNA polymerase II activity is essential for terminal erythroid maturation. Blood 2021, 138: 1740-1756. PMID: 34075391, PMCID: PMC8569412, DOI: 10.1182/blood.2020009903.Peer-Reviewed Original ResearchConceptsRNA polymerase IIRNA polymerase II activityTerminal erythroid maturationPolymerase II activityPolymerase IIErythroid maturationHuman erythroblastsGene expressionTerminal maturationII activityStage-specific regulationHistone posttranslational modificationsTransposase-accessible chromatinErythroid-specific genesAccumulation of heterochromatinHigh-throughput sequencingLevel of transcriptionLate-stage erythroblastsEssential biologic processesAccessible chromatinHistone marksTranscription elongationChromatin structureTranscriptional repressionChromatin immunoprecipitation
1989
RNA Polymerase II Subunit RPB4 Is Essential for High- and Low-Temperature Yeast Cell Growth
Woychik N, Young R. RNA Polymerase II Subunit RPB4 Is Essential for High- and Low-Temperature Yeast Cell Growth. Molecular And Cellular Biology 1989, 9: 2854-2859. DOI: 10.1128/mcb.9.7.2854-2859.1989.Peer-Reviewed Original ResearchRPB4 subunitRNA polymerase II subunitEscherichia coli RNA polymeraseSequence analysis of tryptic peptidesAmino acid sequence analysis of tryptic peptidesRNA polymerase II activityAmino acid sequence analysisDeletion of RPB4Polymerase II subunitAnalysis of tryptic peptidesRNA polymerase IIAmino acidsPolymerase II activityRPB4 geneSigma subunitCrude extracts in vitroPolymerase IIRNA polymeraseSaccharomyces cerevisiaeEnzyme assemblyDNA sequencesRpb4Purified subunitsExtracts in vitroMRNA synthesis
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