2019
In vivo CRISPR screening in CD8 T cells with AAV–Sleeping Beauty hybrid vectors identifies membrane targets for improving immunotherapy for glioblastoma
Ye L, Park JJ, Dong MB, Yang Q, Chow RD, Peng L, Du Y, Guo J, Dai X, Wang G, Errami Y, Chen S. In vivo CRISPR screening in CD8 T cells with AAV–Sleeping Beauty hybrid vectors identifies membrane targets for improving immunotherapy for glioblastoma. Nature Biotechnology 2019, 37: 1302-1313. PMID: 31548728, PMCID: PMC6834896, DOI: 10.1038/s41587-019-0246-4.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDCD8-Positive T-LymphocytesCell Line, TumorCRISPR-Cas SystemsDependovirusFemaleGene EditingGlioblastomaHumansImmunotherapy, AdoptiveLymphocyte Activation Gene 3 ProteinMaleMembrane ProteinsMiceN-AcetylglucosaminyltransferasesNeoplasm ProteinsProtein Disulfide-IsomerasesReceptors, Cell SurfaceRNA, Guide, CRISPR-Cas SystemsTransposasesXenograft Model Antitumor AssaysConceptsRNA cassetteMembrane protein targetsPrimary murine T cellsGenetic screening systemSingle-cell sequencingScreen hitsSleeping Beauty (SB) transposonCRISPR screensMembrane proteinsCell sequencingT cellsAdeno-associated virusGenomic integrationMembrane targetsMurine T cellsProtein targetsEfficient geneHuman GBM cellsGene editingT cell receptor transgenic modelGBM cellsBeauty transposonPDIA3T cell-based immunotherapyAntigen-specific killing
2002
A Non-class I MHC Intestinal Epithelial Surface Glycoprotein, gp180, Binds to CD8
Campbell N, Park M, Toy L, Yio X, Devine L, Kavathas P, Mayer L. A Non-class I MHC Intestinal Epithelial Surface Glycoprotein, gp180, Binds to CD8. Clinical Immunology 2002, 102: 267-274. PMID: 11890713, DOI: 10.1006/clim.2001.5170.Peer-Reviewed Original ResearchMeSH KeywordsAbsorptionAdaptor Proteins, Signal TransducingAnti-Bacterial AgentsAntibodies, MonoclonalBlotting, WesternCarcinoma, HepatocellularCarrier ProteinsCD40 AntigensCD8-Positive T-LymphocytesEnterocytesEnzyme-Linked Immunosorbent AssayEpitopesExtracellular Matrix ProteinsHumansImmediate-Early ProteinsLiver NeoplasmsMembrane GlycoproteinsPhosphorylationProteinsRecombinant Fusion ProteinsSequestosome-1 ProteinTransfectionTumor Cells, CulturedTunicamycinConceptsActivation of CD8T cellsIntestinal epithelial cellsCD8 moleculesEpithelial cellsMucosal immune responsesClass I MHCNormal intestinal epithelial cellsT cell activationMurine T cellsCD8 alphaImmune responseCytolytic activityI MHCCell activationMixed cell culture systemCD8Human CD8 alphaMonoclonal antibodiesCell culture systemActivationSurface glycoproteinTyrosine kinaseCellsGp180
1996
Induction of Functional Empty Class I Major Histocompatibility Complex Glycoproteins by Photoactivated 8-Methoxypsoralen
Imaeda S, Felli A, Schmitt I, Chimenti S, Edelson R. Induction of Functional Empty Class I Major Histocompatibility Complex Glycoproteins by Photoactivated 8-Methoxypsoralen. Journal Of Investigative Dermatology 1996, 107: 887-890. PMID: 8941680, DOI: 10.1111/1523-1747.ep12331193.Peer-Reviewed Original ResearchConceptsMHC class IClass IClass I major histocompatibility complex glycoproteinsAntigenic peptidesLow-dose ultravioletMajor histocompatibility complex glycoproteinsT cell receptor recognitionExperimental animal tumorsClass I moleculesMurine T cellsSpecific CD8CTL responsesT cellsLymphocytes bindMalignant cellsPeptide antigensAnimal tumorsTumor cellsPoor inducerI moleculesCD8Receptor recognitionRetained functionCellsPeptides
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