2010
Enhanced BRET Technology for the Monitoring of Agonist-Induced and Agonist-Independent Interactions between GPCRs and β-Arrestins
Kocan M, Dalrymple M, Seeber R, Feldman B, Pfleger K. Enhanced BRET Technology for the Monitoring of Agonist-Induced and Agonist-Independent Interactions between GPCRs and β-Arrestins. Frontiers In Endocrinology 2010, 1: 12. PMID: 22654789, PMCID: PMC3356007, DOI: 10.3389/fendo.2010.00012.Peer-Reviewed Original ResearchBioluminescence resonance energy transferG protein-coupled receptorsVariant of Renilla luciferaseMonitoring of protein-protein interactionsProtein-protein interactionsBioluminescent donor enzymeVasopressin type II receptorHeterologous co-expressionProtein-protein complexesReal-time monitoring of protein-protein interactionsBioluminescence resonance energy transfer technologyB-arrestinAgonist-independent mannerDonor enzymeBioluminescence resonance energy transfer methodsFusion proteinResonance energy transferBRET technologyMonitor interactionsCoelenterazine substrateRenilla luciferaseAcceptor fluorophoresMutant receptorsType II receptorLiving cells
2009
Conformational transitions in DNA polymerase I revealed by single-molecule FRET
Santoso Y, Joyce CM, Potapova O, Le Reste L, Hohlbein J, Torella JP, Grindley ND, Kapanidis AN. Conformational transitions in DNA polymerase I revealed by single-molecule FRET. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 107: 715-720. PMID: 20080740, PMCID: PMC2818957, DOI: 10.1073/pnas.0910909107.Peer-Reviewed Original ResearchConceptsDNA polymerase IClosed conformationPolymerase IConformational transitionSingle-molecule fluorescence resonance energy transferEarly stepsSingle-molecule FRETFluorescence resonance energy transferAvailable crystallographic structuresResonance energy transferMost DNA polymerasesComplementary ribonucleotidesChemical stepIncorrect substratesPolymerase moleculesPol DNAReaction pathwaysAcceptor fluorophoresKinetic checkpointsConformational dynamicsConformational flexibilityNucleotide additionStructural studiesDNA polymeraseCrystallographic structure
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