2023
Chemical labeling and proteomics for characterization of unannotated small and alternative open reading frame-encoded polypeptides
Chen Y, Cao X, Loh K, Slavoff S. Chemical labeling and proteomics for characterization of unannotated small and alternative open reading frame-encoded polypeptides. Biochemical Society Transactions 2023, 51: 1071-1082. PMID: 37171061, PMCID: PMC10317152, DOI: 10.1042/bst20221074.Peer-Reviewed Original ResearchConceptsChemical labelingAlternative open reading framesOpen reading frameLimited sequence homologyMammalian genomesGene classesProtein domainsQuantitative proteomicsReading frameSequence homologyBiological roleProteomic discoveryMolecular levelSmORFsCell proliferationProteomicsInteractomeGenomeSpecific propertiesExperimental techniquesLabelingHomologyPolypeptideProteinFunctionalization
1986
Two RNA species co‐purify with RNase P from the fission yeast Schizosaccharomyces pombe.
Krupp G, Cherayil B, Frendewey D, Nishikawa S, Söll D. Two RNA species co‐purify with RNase P from the fission yeast Schizosaccharomyces pombe. The EMBO Journal 1986, 5: 1697-1703. PMID: 3743551, PMCID: PMC1166996, DOI: 10.1002/j.1460-2075.1986.tb04413.x.Peer-Reviewed Original ResearchConceptsM1 RNARNA speciesK RNASecondary structureFission yeast SchizosaccharomycesRNase P activityYeast genomic DNALimited sequence homologyYeast SchizosaccharomycesHaploid genomeSchizosaccharomyces pombeRNase PSingle copySouthern analysisSequence homologyGenomic DNAP activityRNAEscherichia coliHairpin loopSame basic organizationEnzyme activityBasic organizationInactivation experimentsSpecies
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