Impact of high temperatures on enzyme-linked immunoassay (ELISA) performance for leptin measurements in human milk stored under varied freeze/thaw conditions
Bertacchi V, Corley M, Aronsen G, Bribiescas R. Impact of high temperatures on enzyme-linked immunoassay (ELISA) performance for leptin measurements in human milk stored under varied freeze/thaw conditions. PLOS ONE 2025, 20: e0320366. PMID: 40106448, PMCID: PMC11957766, DOI: 10.1371/journal.pone.0320366.Peer-Reviewed Original ResearchConceptsMilk samplesHigh ambient temperature exposureExposure to warm temperaturesWarmer temperaturesHuman milk samplesImpact of high temperatureNormal kitsRecommended temperatureAssay performance validationEnzyme-linked immunoassayAmbient temperature exposureAmbient temperature conditionsHigh temperatureTemperature conditionsELISA kitsTemperature exposureLeptin measurementsShipping conditionsLaboratory analysis
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