1998
Electrophysiology in the eukaryotic model cell Saccharomyces cerevisiae
Bertl A, Bihler H, Kettner C, Slayman CL. Electrophysiology in the eukaryotic model cell Saccharomyces cerevisiae. Pflügers Archiv - European Journal Of Physiology 1998, 436: 999-1013. PMID: 9799419, DOI: 10.1007/s004240050735.Peer-Reviewed Original ResearchConceptsYeast plasma membranePlasma membraneMembrane proteinsYeast membranesFunctional analysisHeterologous membrane proteinsFacile genetic manipulationDetailed functional analysisPreliminary functional analysisYeast vacuolar membraneGigaseal formationHeterologous genesYeast vacuoleCytoplasmic proteinsVacuolar membraneFungal proteinsGenetic manipulationSpecific proteinsPolyploid strainsSaccharomycesNative proteinYeastProtoplastsIon channelsProtein
1992
Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants
Jones J, Shlumukov L, Carland F, English J, Scofield S, Bishop G, Harrison K. Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants. Transgenic Research 1992, 1: 285-297. PMID: 1338696, DOI: 10.1007/bf02525170.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid OxidoreductasesBase SequenceCloning, MolecularDNA Transposable ElementsDrug ResistanceEscherichia coliGenetic TechniquesGenetic VectorsGlucuronidaseMolecular Sequence DataOligodeoxyribonucleotidesPlantsPlants, Genetically ModifiedPlasmidsPromoter Regions, GeneticProtein Sorting SignalsRestriction MappingTransformation, GeneticConceptsTransgenic plantsHeterologous genesNopaline synthaseCauliflower mosaic virus 35S promoterΒ-glucuronidase gene expressionPlant cell transformationActivity of transposonsPlant molecular biologyBinary vector constructsSelection marker geneTransgene of interestPlant transformationHeterologous expressionSynthase promoterEffective vectorPolyadenylation sequenceReading frameTransposon excisionMarker genesGene expressionCell transformationMolecular biologyVector constructsGenesPlasmid
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