Arabidopsis thaliana Nfu2 Accommodates [2Fe-2S] or [4Fe-4S] Clusters and Is Competent for in Vitro Maturation of Chloroplast [2Fe-2S] and [4Fe-4S] Cluster-Containing Proteins
Gao H, Subramanian S, Couturier J, Naik S, Kim S, Leustek T, Knaff D, Wu H, Vignols F, Huynh B, Rouhier N, Johnson M. Arabidopsis thaliana Nfu2 Accommodates [2Fe-2S] or [4Fe-4S] Clusters and Is Competent for in Vitro Maturation of Chloroplast [2Fe-2S] and [4Fe-4S] Cluster-Containing Proteins. Biochemistry 2013, 52: 6633-6645. PMID: 24032747, PMCID: PMC3819817, DOI: 10.1021/bi4007622.Peer-Reviewed Original ResearchConceptsYeast two-hybrid studiesIron-sulfur (Fe-SAdenosine 5'-phosphosulfate reductaseChloroplastic Fe-S proteinsReductive sulfur assimilationTwo-hybrid studiesBiosynthesis of cysteineFe-S cluster incorporationCombination of UV-visible absorptionNfu proteinsArabidopsis thalianaNFU2CXXC motifSulfur assimilationPlant metabolismFe proteinCluster transferGel filtration studiesArabidopsisFe-S clustersProteinGlutaredoxinUV-visible absorptionCysteineCluster incorporation
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