Substrate Profiling of Cysteine Proteases Using a Combinatorial Peptide Library Identifies Functionally Unique Specificities*
Choe Y, Leonetti F, Greenbaum DC, Lecaille F, Bogyo M, Brömme D, Ellman JA, Craik CS. Substrate Profiling of Cysteine Proteases Using a Combinatorial Peptide Library Identifies Functionally Unique Specificities*. Journal Of Biological Chemistry 2006, 281: 12824-12832. PMID: 16520377, DOI: 10.1074/jbc.m513331200.Peer-Reviewed Original ResearchConceptsIndividual peptide substratesSubstrate specificitySpecificity profilePeptide substratesSelective chemical probesPositional scanning synthetic combinatorial librariesStandard sequence analysisSimilar substrate specificityAmino acid preferencesSubstrate-based inhibitorsSynthetic combinatorial librariesCysteine protease papainThree-dimensional structureCleavage site sequenceFunctional characterizationChemical probesCoumarin fluorophoreFacilitated synthesisHuman cathepsin LPhysiological substratesSubstrate sequenceSite sequenceSequence analysisCysteine proteasesCombinatorial libraries
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